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目的观察涤痰汤对帕金森病(PD)药物左旋多巴(LD)毒副反应大鼠纹状体多巴胺转运蛋白(DAT)的影响。方法在PD模型大鼠的基础上腹腔注射左旋多巴/苄丝肼2周制备LD模型,将成功LD模型大鼠随机分为LD模型组、涤痰汤组各9只,另取正常对照组和PD模型对照组大鼠各9只。LD模型组、涤痰汤组分别给予生理盐水、涤痰汤灌胃;PD模型对照组、正常对照组给予生理盐水灌胃(分别为2、4、6周)。每组在2、4、6周各取3只大鼠,行脑纹状体内DAT125I-β-CIT放射自显影,同时用γ-计数器测定DAT放射活性。结果 LD模型组随时间延长DAT数量有减少趋势,且在4、6周时均较PD模型对照组为低;涤痰汤组随干预时间的延长,DAT数量有增加趋势,且在4、6周时均较LD模型组为高,但差异均无统计学意义(P>0.05)。自显影结果与DAT变化基本一致。结论涤痰汤可以缓解帕金森病左旋多巴治疗引起的DAT数量减少。
Objective To observe the effect of Ditan Decoction on dopamine transporter (DAT) in the striatum of rats with Parkinson’s disease (PD) -induced levodopa (LD) toxicity. Methods LD model was prepared by intraperitoneal injection of levodopa / benserazide on the basis of PD model rats. LD model rats were randomly divided into LD model group and Ditan Decoction group, each group received 9 rats. Another group of normal control group And PD model control group, 9 rats each. LD model group and Ditan group were administered with normal saline and Ditan Decoction respectively. PD model control group and normal control group were given normal saline (2,4,6 weeks respectively). DAT125I-β-CIT autoradiography was performed in brain striatum of 3 rats in each group at 2, 4 and 6 weeks. The radioactivity of DAT was measured by γ-counter. Results The number of DAT in the LD model group decreased with time and was lower than that in the PD model control group at 4 and 6 weeks. The number of DAT increased with the prolongation of intervention time in Ditan Decoction group, and it increased at 4 and 6 weeks LD model group was high, but the difference was not statistically significant (P> 0.05). Self-imaging results and DAT changes are basically the same. Conclusion Ditan decoction can relieve the decrease of DAT caused by Parkinson’s disease levodopa treatment.