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目的探讨HPV L1壳蛋白与p16INK4A联合检测在宫颈上皮内瘤变(CIN)中的表达及临床意义。方法选择经薄层液基细胞学(TCT)及宫颈活检检查患者226例,根据宫颈活检病理诊断分为炎症组、CIN组、鳞形细胞癌(SCC)组。TCT标本检测HPV L1壳蛋白的表达,宫颈活检标本检测p16INK4A的表达,将检测结果行相关分析。结果病理分级与HPV L1壳蛋白阳性率呈负相关(P<0.01),与p16INK4A阳性率呈正相关(P<0.01)。与SCC组比较,CIN组HPV L1壳蛋白阳性率明显升高,而p16INK4A阳性率明显降低(P<0.01,P<0.05)。结论 HPV L1壳蛋白与p16INK4A均可作为CIN辅助诊断的生物标志物。联合检测时,HPV L1(+)/p16INK4A(-)提示宫颈病变无进展或有消退的可能。
Objective To investigate the expression of HPV L1 capsid protein and p16INK4A in cervical intraepithelial neoplasia (CIN) and its clinical significance. Methods Totally 226 patients underwent TCT and cervical biopsy were divided into inflammatory group, CIN group and squamous cell carcinoma (SCC) group according to the pathological diagnosis of cervical biopsy. The expression of HPV L1 capsid protein was detected by TCT and the expression of p16INK4A was detected by cervical biopsy specimens. The results were analyzed by correlation analysis. Results The pathological grade was negatively correlated with the positive rate of HPV L1 capsid protein (P <0.01), and positively correlated with the positive rate of p16INK4A (P <0.01). Compared with SCC group, the positive rate of HPV L1 capsid protein in CIN group was significantly increased, while the positive rate of p16INK4A was significantly decreased (P <0.01, P <0.05). Conclusion Both HPV L1 capsid protein and p16INK4A can be used as biomarkers for the diagnosis of CIN. When tested together, HPV L1 (+) / p16INK4A (-) suggests that cervical lesions may not progress or recede.