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目的研究人MUC1/Y基因转染人外周血树突状细胞(DC)后在体外诱导的特异性抗MUC1/Y+卵巢癌效应。方法通过人外周血单核细胞诱导DC,采用脂质体法将已构建好的含人MUC1/Y全长cDNA的真核表达载体pEGFPN1/MUC1/Y转染到DC中,荧光显微镜下观察其表达的蛋白定位;与自体T细胞共培养,观察其致敏的细胞毒性T淋巴细胞(CTL)对MUC1/Y+的卵巢癌细胞A2780的杀伤活性,并与转染空载体的DC诱导的CTL进行比较。结果pEGFPN1/MUC1/Y转染DC后,其绿色荧光蛋白主要表达于DC细胞膜上,可诱导出对卵巢癌细胞系A2780特异性的细胞毒性T细胞。结论MUC1/Y基因修饰的DC可诱导特异的抗MUC1/Y+卵巢癌效应。
Objective To investigate the specific anti-MUC1 / Y + ovarian cancer-inducing effect of human MUC1 / Y gene transfected human peripheral blood dendritic cells (DCs) in vitro. Methods DCs were induced by human peripheral blood mononuclear cells. The constructed eukaryotic expression vector pEGFPN1 / MUC1 / Y containing human MUC1 / Y cDNA was transfected into DCs by lipofectamine. And the coculture of autologous T cells to observe the cytotoxicity of sensitized cytotoxic T lymphocytes (CTLs) to MUC1 / Y + -positive ovarian cancer cells A2780, and the results were compared with DC-induced CTLs transfected with empty vector Compare Results After transfection of DCs with pEGFPN1 / MUC1 / Y, their green fluorescent protein was mainly expressed on the membrane of DC and induced cytotoxic T cells specific to ovarian cancer cell line A2780. Conclusion MUC1 / Y gene-modified DC can induce specific anti-MUC1 / Y + ovarian cancer effect.