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探讨不同ＤＮＡ源作基因多态性分析的优缺点，及以血凝块裂解液直接进行ＰＣＲ扩增的可行性 和可靠性。方法：从口腔粘膜细胞、组织切片提取ＤＮＡ，并将临床生化分析遗弃的血凝块制备成裂解液和纯化 ＤＮＡ，取各自样本分别进行ＰＣＲ扩增，并进一步对血凝块纯化ＤＮＡ和血凝块裂解液所作的ＰＣＲ扩增作对照研究。 结果：本研究使用的不同ＤＮＡ源所作的ＰＣＲ扩增均获得满意结果，以血凝块裂解液进行的ＧＳＴＭ１，ＧＳＴＴ１和 ＣＹＰ２Ｅ１基因多态性分析均获得成功，其结果与纯化ＤＮＡ进行分析观察到的结果完全一致。结论：同组织细胞纯 化的ＤＮＡ一样，血凝块是理想的ＤＮＡ源，用血凝块裂解液直接进行基因多态性分析，方法简明，结果可靠。
The advantages and disadvantages of genetic polymorphism analysis of different DNA sources were explored, as well as the feasibility and reliability of direct PCR amplification with clot lysates. Methods: DNA was extracted from oral mucosal cells and tissue sections, and the blood clots that were abandoned in clinical biochemical analysis were prepared into lysates and purified DNA. Each sample was taken for PCR amplification, and further purified DNA and blood clots were obtained for clot clotting. PCR amplification of block lysates was used as a control study. Results: The PCR amplification results from different DNA sources used in this study were satisfactory. The results of GSTM1, GSTT1, and CYP2E1 polymorphism analysis using the clot lysate were successful. The results were analyzed with purified DNA. The results are exactly the same. Conclusion: As with DNA purified from tissue cells, blood clots are an ideal source of DNA. Genetic polymorphism analysis using clot lysates is straightforward and results are reliable.