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[Objective] The aim of this study was to evaluate the nutritive value of Takifugu obscurus and provide the scientific basis for artificially feed research. [Method] The nutrient components and nutritive qualities in the muscle of wild and artificially reared Takifugu obscurus were comparatively analyzed. [Result] The contents of crude protein, crude fat, crude ash, total amino acids, essential amino acids and half essential amino acid in the muscle of wild Takifugu obscurus were all higher than those of the artificially reared. The content of four delicious amino acids in the muscle of wild and artificially reared Takifugu obscurus was 17.59% and 14.44% (percentage of dry weight), respectively. The essential amino acid index (EAAI) was 52 and 40 separately, and the constitutional rate of the essential amino acids met the FAO/WHO standard. Valine was the first limiting amino acid of Takifugu obscurus. [Conclusion] The muscle of wild and artificially reared Takifugu obscurus were all high quality food full of animal protein, while the protein nutritive value of wild Takifugu obscurus was higher than that of the artificially reared.