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Bentwich认为神经氨酸酶处理正常人PBL后可使E花结率上升到94％,推测有一个B细胞亚群在酶的影响下能表现出E受体。Chapel认为E受体是对胰酶敏感的糖蛋白分子,用胰酶可脱下E受体,而不影响E受体的活性。我们用胰酶脱E受体发现非常小剂量的胰酶相反能提高E花结率。本试验用一系列倍量稀释的胰酶与神经氨酸酶处理PBL,选出使E花结增高的最适浓度,进而用以对尼龙毛过滤的非粘附与粘附细胞分别处理,再做E_(t(AET))-C_3混合花结测定,以了解提高的E花结部分是来自哪一类细胞群。
Bentwich believes that neuraminidase treatment of normal PBL can make E flowering rate increased to 94%, presumably a B cell subsets under the influence of the enzyme can show the E receptor. Chapel believes that the E receptor is a pancreatin-sensitive glycoprotein molecule that can be detached with trypsin without affecting the activity of the E receptor. We used trypsin to remove E receptors and found that very small doses of trypsin conversely increased E-stature. In this experiment, PBL was treated with a series of diluted trypsin and neuraminidase to select the optimal concentration for increasing the E-knot, which was then used to treat the non-adherent and adherent cells of the nylon wool filter separately The E_ (t (AET)) - C_3 mixed rosetting assay was performed to see which cell population the increased E-rosettes came from.