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目的:通过复制酒精性更年期综合征大鼠模型,观察二仙汤及大豆黄酮对该模型大鼠卵巢形态学及功能的影响,探讨两种药物对绝经综合征症状改善的机理。方法:卵巢注射无水乙醇法制备绝经综合征SD大鼠模型,以戊酸雌二醇作为阳性对照药,测定血清E2(雌二醇)及FSH(促卵泡生成激素)含量,肉眼及HE染色显微镜下观察卵巢的形态学改变,并检测卵巢雌激素受体β。结果:灌胃4周后,除模型组外,各组体重均增加。二仙汤组与模型组比较,SD大鼠血清E2水平明显升高,差异有统计学意义(P<0.01)。二仙汤组、大豆黄酮组与模型组SD大鼠血清FSH水平比较,差异均无统计学意义(P>0.05)。肉眼及HE染色观察卵巢与子宫结果示:除假手术组外,均发生明显异常;二仙汤组卵巢部分组织有成熟卵泡、囊腔有新生组织出现;大豆黄酮组卵巢初级、次级卵泡较多,囊腔有大量新生组织出现,子宫结缔组织致密。各组卵巢雌激素受体β比较,差异均无统计学意义(P>0.05)。结论:二仙汤能明显升高绝经综合征大鼠模型血清E2水平,促进卵巢卵泡成熟及囊腔新生组织出现;大豆黄酮能促使卵巢初级、次级卵泡增多及囊腔新生组织大量出现,增加子宫结缔组织致密性。本研究为二仙汤、大豆黄酮对绝经综合征症状改善提供理论基础。
OBJECTIVE: To observe the effects of Erxian Decoction and daidzein on morphological and functional changes of ovary in rats with the model of climacteric syndrome by observing the effects of Erxian Decoction and daidzein on the ovarian morphology and the mechanism of the improvement of the symptoms of menopausal syndrome. Methods: SD rat model of menopause syndrome was established by injecting ethanol into the ovary. The estradiol valerate was used as the positive control. Serum E2 (estradiol) and FSH (follicle stimulating hormone) were measured. Gross and HE staining The morphological changes of the ovary were observed under a microscope, and the estrogen receptor β of the ovary was detected. Results: After 4 weeks of gavage, the body weights of all groups except the model group increased. Erxian decoction group compared with the model group, serum E2 level of SD rats increased significantly, the difference was statistically significant (P <0.01). There was no significant difference in serum FSH levels between Erxiantang group, daidzein group and model group (P> 0.05). The naked eye and HE staining showed ovarian and uterine results showed that except for the sham operation group, significant abnormalities occurred; some ovarian tissues of Erxian decoction group had mature follicles and new tissue appeared in the cyst cavity; the primary and secondary follicles of daidzein group More, there are a large number of cysts nascent tissue, uterine connective tissue density. There was no significant difference in the estrogen receptor β in each group (P> 0.05). Conclusion: Erxiantang can significantly increase the level of serum E2 in rat model of menopause syndrome, and promote the ovarian follicles to mature and the appearance of cystic cavity; daidzein can promote the ovarian primary and secondary follicles increased and a large number of cystic neovascularization, increased Uterine connective tissue density. This study is Erxian decoction, daidzein on menopausal syndrome symptoms provide a theoretical basis.