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本文对不同培养基、不同外植体类型(节点、节间、下胚轴、半粒种子、果段)诱导哈瓦那辣椒产生体细胞胚胎的情况进行了评估。在添加9.05μM 2,4-D、3%蔗糖和0.8%植物凝胶的MS基本培养基中,25±2℃,连续30 d光照(40~50μmol·m2/s)条件下,诱导胚性愈伤组织。形成的愈伤组织将被转移到组成相同的液体培养基中。仅当以下胚轴外植体,用含3.4μM噻苯隆的MS培养基对其进行诱导时,才能产生体细胞胚。本研究建立了辣椒“细胞悬浮培养的体细胞胚发生”的一种实验方案。
In this paper, we evaluated the induction of somatic embryogenesis by Havana pepper in different media and with different explant types (nodes, internodes, hypocotyls, half seeds, and fruit segments). Embryogenicity was induced in MS basal medium supplemented with 9.05μM 2,4-D, 3% sucrose and 0.8% phytogel at 25 ± 2 ° C for 30 d light exposure (40-50 μmol · m2 / s) Callus. The callus formed will be transferred to the same liquid medium. Somatic embryos can only be produced when the following hypocotyl explants are induced with 3.4 [mu] M thidiazuron in MS medium. This study established a pepper “cell suspension culture of somatic embryo ” an experimental program.