论文部分内容阅读
目的研究颈髓S-100蛋白分布及半切损伤后S-100蛋白的分布变化及时相改变。探索其与恢复的关系。方法通过免疫组织化学技术观察大鼠颈脊髓半切伤综合征(Brown-sequard’ssydrone)不同时期神经组织中S-100蛋白的分布变化。结果在未损伤的颈髓神经中,S-100蛋白分布于雪旺细胞的胞桨和胞膜中。神经切断48小时后,远近节段S-100蛋白反应表达减弱,远端1周后几乎没有S-100的表达,而近段有新生轴索生长时重新表达S-100蛋白。结论轴索对于雪旺细胞的成熟具有重要作用,S-100蛋白对于轴索的再生具有刺激和诱导作用。S-100蛋白在颈髓不只存在于成熟的雪旺细胞,而且存在于胶质细胞中。S-100蛋白的出现预示着雪旺细胞的成熟和神经再生的表现。
Objective To study the distribution of S-100 protein and the change of time phase after S-100 protein distribution and semi-incision injury in cervical spinal cord. Explore its relationship with recovery. Methods Immunohistochemistry was used to observe the distribution of S-100 protein in nerve tissue of different stages of Brown-sequard’ssydrone in rats with cervical spinal cord injury. Results In intact cervical spinal nerves, S-100 protein was distributed in the oocyte and membrane of Schwann cells. After 48 hours of nerve transection, S-100 protein expression in distal and proximal segments decreased. There was almost no expression of S-100 in the distal one week, while S-100 protein was re-expressed in the proximal segment with axonal growth. Conclusion The axonal plays an important role in the maturation of Schwann cells. The S-100 protein stimulates and induces axonal regeneration. The S-100 protein is present not only in mature Schwann cells but also in glial cells in the cervical cord. The presence of S-100 protein predicts the performance of Schwann cells in maturation and neuroregeneration.