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目的:探讨喉鳞状细胞癌(LSCC)组织中癌基因AKT2的表达及临床意义。方法:采用免疫组织化学Elivision二步法检测60例LSCC组织、28例癌旁组织及16例正常喉黏膜标本中AKT2蛋白的表达并分析其与LSCC临床病理特征的关系。结果:AKT2在LSCC和癌旁组织中的阳性表达率分别为56.7%(34/60)和14.3%(4/28),在正常喉黏膜组织中未见表达,LSCC组织中的阳性表达率高于癌旁组织及正常喉黏膜组织(均P<0.05);AKT2的表达与LSCC发生部位、组织学分级、淋巴结转移、临床分期密切相关(均P<0.05),而与患者年龄、性别、吸烟、T分期均无相关性(均P>0.05)。结论:AKT2在LSCC的发生、发展中可起重要作用,可作为预测LSCC发生及侵袭转移潜能的参考指标之一。
Objective: To investigate the expression of oncogene AKT2 in laryngeal squamous cell carcinoma (LSCC) and its clinical significance. Methods: Immunohistochemical Elivision two-step method was used to detect the expression of AKT2 protein in 60 LSCC tissues, 28 paracancerous tissues and 16 normal laryngeal mucosa specimens. The relationship between them and clinicopathological features of LSCC was analyzed. Results: The positive rates of AKT2 expression in LSCC and adjacent tissues were 56.7% (34/60) and 14.3% (4/28) respectively, which were not found in normal laryngeal mucosa. The positive rate of AKT2 in LSCC tissues was high (P <0.05). The expression of AKT2 was closely related to the location, histological grade, lymph node metastasis and clinical stage of LSCC (all P <0.05), but not with age, gender, smoking status , T stage were not related (all P> 0.05). Conclusion: AKT2 may play an important role in the occurrence and development of LSCC, which may be used as a reference index for predicting the occurrence and metastasis potential of LSCC.