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泰国各地采到带有一种不知名的病害和其他几种病毒病症状的水稻植株。用电镜和浸渍法从该植株上测定出有几种病毒病的存在。研究结果表明那种不知名的病害不是东格罗感染所引起。从田间有病状植株上采集小的叶片样本(2×2m~2),置试管中,加入含有0.02%pv的0.1M 3—HCL 缓冲液(pH7.2),然后用玻璃棒捣碎。再滴入两滴敏化悬浮乳胶与抗血清并摇动30分钟,静置2小时后观察。在阳性反应中乳胶粒子凝聚,在阴性反应中则其仍呈
Rice plants in Thailand have been spotted with an unknown disease and several other viral disease symptoms. The presence of several viral diseases was determined from the plants by electron microscopy and dipping. The results show that the unknown disease is not caused by infection with tunggro. Small leaf samples (2 x 2 m ~ 2) were collected from diseased plants in the field and placed in test tubes. 0.1 M 3-HCL buffer (pH 7.2) containing 0.02% pv was added and then mashed with a glass rod. Two drops of sensitized suspension latex and antiserum were then added dropwise and shaken for 30 minutes and allowed to stand for 2 hours before observation. The latex particles agglutinate in a positive reaction, while they remain in a negative reaction