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以3-[N,N-二甲基-[2-(2-甲基丙-2-烯酰氧基)乙基]铵]丙烷-1-磺酸内盐(SPE)为功能单体,季戊四醇三丙烯酸酯(PETA)为交联剂,环己醇和乙二醇(EG)为致孔剂,通过原位聚合法制备磺酸甜菜碱型两性离子亲水毛细管整体柱。优化单体、交联剂和致孔剂的比例等因素,考察了不同SPE含量对整体柱性能和选择性的影响。在最优制备条件下,以苯酚类化合物、烷基苯类化合物和苯甲酸类化合物为分离对象,评价该整体柱的色谱性能以及分离机理。在不同的色谱条件下,该整体柱具有亲水、疏水以及离子交换作用。此整体柱在0.05 m L/min的流速下(线速度为0.265 mm/s)分离烷基苯类化合物时,柱效高达41000~56000 plates/m,该整体柱重现性良好,连续运行的重现性(RSD)低于1.2%。在亲水/离子交换色谱模式下,该整体柱可应用于核苷和碱基的高效分离。
The functional group was synthesized using 3- [N, N-dimethyl- [2- (2-methylprop-2- enoyloxy) ethyl] ammonium] propane- Pentaerythritol triacrylate (PETA) as crosslinking agent, cyclohexanol and ethylene glycol (EG) as pore-forming agents were prepared by in-situ polymerization of sulfobetaine-type zwitterionic hydrophilic capillary monolithic columns. Optimize the ratio of monomer, crosslinking agent and porogen and other factors, the effect of different SPE content on the performance and selectivity of the monolithic column were investigated. Under the optimal preparation conditions, the chromatographic performance and separation mechanism of the monolithic column were evaluated by separating phenolic compounds, alkylbenzenes and benzoic acids. Under different chromatographic conditions, the monolith has hydrophilic, hydrophobic and ion exchange effects. The monolithic column separates alkylbenzenes at a flow rate of 0.05 m L / min (linear velocity of 0.265 mm / s) with column efficiencies of up to 41,000 to 56,000 plates / m with good reproducibility of the monolithic column Reproducibility (RSD) is less than 1.2%. In hydrophilic / ion exchange chromatography mode, the monolithic column can be applied to the efficient separation of nucleosides and bases.