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以大青山阳坡柄扁桃幼根及根围土壤为试材,采用Phillips和Hayman的染色、湿筛倾析-蔗糖离心及土壤理化因子常规测定方法,研究了不同坡位柄扁桃根围土壤丛枝菌根真菌(AMF)群落结构及其土壤理化因子,以期了解土壤理化因子对柄扁桃根围土壤AMF群落结构的影响。结果表明:不同坡位柄扁桃菌根侵染率在一定范围内随着海拔的升高呈先升高后降低的现象。在不同坡位柄扁桃根围土壤中共分离出12属33种AMF,其中Funneliformis mosseae和Rhizophagus intraradices属于优势种。下坡位和中坡位AMF的孢子密度显著高于上坡位(P<0.05)。下坡位种的丰度和Shannon-Weiner指数均显著高于中坡位和上坡位(P<0.05)。AMF多样性主要受土壤理化因子全磷、pH、速效钾、速效磷的影响。
Using the methods of Phillips and Hayman staining, wet-sieving decantation-sucrose centrifugation and physicochemical determination of soil, we studied the effects of different soil types AMF community structure and its soil physico-chemical factors in order to understand the effects of soil physico-chemical factors on the AMF community structure in the stems and shoots. The results showed that the infection rates of the mycorrhizal fungi in different slopes increased firstly and then decreased with the altitude increasing within a certain range. A total of 33 species of AMF were isolated from 12 genera of almonds in different slopes. Funneliformis mosseae and Rhizophagus intraradices were the dominant species. The spore density of AMF in downhill and mid-slope was significantly higher than that in uphill (P <0.05). The abundance and Shannon-Weiner index of downhill species were significantly higher than those of mid-slope and up-slope (P <0.05). AMF diversity is mainly affected by soil physical and chemical factors of total phosphorus, pH, available potassium, available phosphorus.