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目的:为了使培养细胞的遗传性状、生物学特性相似,利于多种实验研究,而对视网膜母细胞瘤SO-Rb_(50)细胞系进行克隆。方法:用多孔塑料板克隆细胞法,对SO-Rb_(50)细胞进行克隆及分离培养。结果:没有菊花团形成的Rb细胞克隆成功,而有菊花团的Rb细胞、连续三次克隆均不能成功。结论:提示分化好的Rb细胞难以克隆成功。眼科学报 1996;12:79—82。
OBJECTIVE: To clone and characterize the retinoblastoma SO-Rb_ (50) cell line in order to make the genetic characters and biological characteristics of the cultured cells similar and to facilitate a variety of experimental studies. Methods: SO-Rb_ (50) cells were cloned and isolated by using the porous plastic plate cloning method. RESULTS: Rb cells cloned without chrysanthemum formation were successfully cloned, whereas chrysanthemum Rb cells were successfully cloned in three consecutive passages. Conclusion: It suggests that the differentiated Rb cells are difficult to clone successfully. Journal of Ophthalmology 1996; 12: 79-82.