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目的研究红景天对大鼠脑缺血再灌注损伤的作用。方法４ＶＯ法复制缺血再灌注动物模型；放免法及化学发光法测定乙酰胆碱（Ａｃｈ）、一氧化氮（ＮＯ）及内皮素（ＥＴ）含量；细胞培养观察红景天对神经细胞的作用。结果（１）缺血再灌注组（ＩＲ）Ａｃｈ含量显著低于假手术组（ＳＡＭ），药物预防后缺血再灌注组（Ｒ＋ＩＲ）及缺血再灌注后药物治疗组（ＩＲ＋Ｒ）较ＩＲ组显著升高。（２）ＩＲ组ＮＯ含量显著高于ＳＡＭ组，Ｒ＋ＩＲ组及ＩＲ＋Ｒ组ＮＯ含量显著降低。（３）ＩＲ组ＥＴ含量显著高于ＳＡＭ组而Ｒ＋ＩＲ组显著降低。（４）红景天甙培养组细胞存活率及ＬＤＨ含量明显高于对照组，ＮＭＤＡ损伤＋红景天甙组细胞存活率明显高于ＮＭＤＡ损伤组，ＬＤＨ含量却明显降低。结论红景天对大鼠脑缺血再灌注损伤时脑神经细胞具有保护作用。
Objective To study the effect of Rhodiola on the cerebral ischemia-reperfusion injury in rats. Methods The 4VO method was used to replicate the model of ischemia / reperfusion. The levels of Ach, NO and ET were determined by radioimmunoassay and chemiluminescence. The effects of Rhodiola rosea on neurons were observed by cell culture. Results (1) The content of Ach in IR group was significantly lower than that in sham group, IR + R group and IR + R group Significantly increased. (2) The content of NO in IR group was significantly higher than that in SAM group, and the content of NO in R + IR group and IR + R group was significantly decreased. (3) ET content in IR group was significantly higher than that in SAM group and R + IR group was significantly decreased. (4) The cell survival rate and LDH content in salidroside culture group were significantly higher than those in control group. The cell viability in NMDA + salidroside group was significantly higher than that in NMDA-injured group, while the LDH content was significantly decreased. Conclusion Rhodiola has neuroprotective effect on cerebral ischemia / reperfusion injury in rats.