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目的探讨头皮位点药物注射对宫内感染早产鼠脑组织髓鞘碱性蛋白(MBP)、胶质纤维酸性蛋白(GFAP)的表达及神经行为学的影响。方法 37只Wistar母鼠于孕16 d、17 d腹腔内注射脂多糖(LPS)或相同剂量的9 g.L-1盐水,将LPS组母鼠早产仔鼠(<孕22 d)于出生第7天分别予头皮位点注射VitB1、VitB12(A组)或丰富的环境干预(B组)或不做干预(C组);各组仔鼠于出生7 d、25 d采用免疫组织化学测脑组织MBP、GFAP蛋白的表达,并进行神经行为学检测。结果 7日龄LPS组早产仔鼠脑组织MBP蛋白表达(112.00±9.27)明显低于9 g.L-1盐水组(124.26±9.40)(P<0.01),大脑皮质及海马齿状回GFAP阳性细胞数目(39.45±4.70;22.55±3.91)明显高于9 g.L-1盐水组(34.36±3.72;18.82±3.25)(Pa<0.05);25日龄仔鼠中,脑组织MBP表达A组(138.79±9.21)高于C组(128.44±12.99)(P<0.01),B组(141.53±13.11)高于C组(P<0.01),GFAP阳性细胞数目大脑皮质B组(45.50±6.54)低于C组(51.42±6.99)(P<0.01),A组(46.70±5.61)低于C组(P<0.05),海马齿状回B组(35.35±3.70)低于C组(38.79±5.56)(P<0.05);悬吊试验得分,A组(3.65±0.22)分、B组(3.60±0.21)分高于C组(2.70±0.21)分(Pa<0.01);旷野试验中的得分,A组得分[(11.20±1.96)分]及B组得分[(10.80±1.96)分]均高于C组[(9.10±1.33)分](Pa<0.01),斜坡试验中B组试验时间[(2.19±0.74)s]最短,A组所用时间[(2.30±0.71)s]明显短于C组[(3.07±0.85)s](P<0.01)。结论宫内感染可诱发孕鼠早产并引起脑组织损伤;头皮位点药物注射可增加大鼠脑组织MBP的表达及抑制GFAP的过度表达,并改善宫内感染早产仔鼠的神经行为。
Objective To investigate the effect of scalp site injection on the expression of MBP and GFAP in premature rat with intrauterine infection. Methods 37 Wistar female rats were injected intraperitoneally with lipopolysaccharide (LPS) or the same dose of 9 gL-1 saline on the 16th day and the 17th day of gestation. The premature pups (<22 days) of LPS group were sacrificed on day 7 The rats in each group were injected with VitB1 and VitB12 (group A) or rich environmental intervention (group B) or no intervention (group C). The pups of each group were detected on the 7th day and 25th day by immunohistochemical method to detect the brain tissue MBP , GFAP protein expression, and conduct neurobehavioral testing. Results The expression of MBP protein in preterm litter of LPS group (112.00 ± 9.27) was significantly lower than that in 9 gL-1 saline group (124.26 ± 9.40) (P <0.01). The number of GFAP positive cells in dentate gyrus of cerebral cortex and hippocampus (39.45 ± 4.70; 22.55 ± 3.91) was significantly higher than that of 9 gL-1 saline group (34.36 ± 3.72; 18.82 ± 3.25) (Pa <0.05) ) Was higher than that of C group (128.44 ± 12.99) (P <0.01), B group (141.53 ± 13.11) was higher than C group (P <0.01), GFAP positive cells in cerebral cortex group B (45.50 ± 6.54) (51.42 ± 6.99) (P <0.01), A group (46.70 ± 5.61) was lower than C group (P <0.05), the hippocampal dentate gyrus group B (35.35 ± 3.70) was lower than C group (38.79 ± 5.56) <0.05). The scores of hanging test in group A (3.65 ± 0.22), group B (3.60 ± 0.21) were higher than those in group C (2.70 ± 0.21) (Pa <0.01) (11.20 ± 1.96) in group B and (10.80 ± 1.96) in group B were significantly higher than those in group C (9.10 ± 1.33) (Pa <0.01) ± 0.74) s]. The time spent in group A [(2.30 ± 0.71) s] was significantly shorter than that in group C (3.07 ± 0.85) s (P <0.01). Conclusion Intrauterine infection can induce premature delivery of pregnant mice and cause brain injury. Scalp site injection can increase the expression of MBP and inhibit the overexpression of GFAP, and improve the neurological behavior of neonatal pups with intrauterine infection.