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目的:探讨β-榄香烯联合顺铂对宫颈癌SiHa细胞生长的影响。方法:体外培养宫颈癌SiHa细胞,分别将β-榄香烯(浓度梯度为25、50、100、150、200μg/ml),顺铂(浓度梯度为1.5、3、6、9、12μg/ml),单独作用于宫颈癌SiHa细胞,加药24h、48h后用CCK-8法检测细胞增殖情况。用流式细胞术检测β-榄香烯组细胞24h凋亡率。选取合适的药物浓度(β-榄香烯125μg/ml,顺铂3μg/ml),进行联合用药,加药24h、48h用CCK-8法检测细胞增殖情况,用流式细胞术检测24h细胞凋亡率。结果:CCK-8法检测显示β-榄香烯和顺铂单独用药24h、48h后,除顺铂1.5μg/ml外,其它实验组对宫颈癌SiHa细胞的抑制率均高于对照组(P<0.05),在一定程度上呈浓度和时间依赖性。联合用药时,细胞的抑制率和凋亡率要显著高于单独用药(P<0.01)。结论:β-榄香烯、顺铂单独或联合作用均能抑制SiHa细胞的增殖,促进其凋亡,且β-榄香烯联合顺铂的作用要显著高于单独用药,β-榄香烯和顺铂可协同(CDI<1)促进SiHa细胞凋亡。
Objective: To investigate the effects of β-elemene and cisplatin on the growth of cervical cancer SiHa cells. Methods: SiHa cells were cultured in vitro. Β-Elemene (concentration gradient 25,50,100,150,200μg / ml), cisplatin (concentration gradient 1.5,3,6,9,12μg / ml ), Acting on cervical cancer SiHa cells alone. After adding drugs for 24h and 48h, cell proliferation was detected by CCK-8 assay. The apoptosis rate of β-elemene cells was detected by flow cytometry. Select the appropriate drug concentration (β-elemene 125μg / ml, cisplatin 3μg / ml) for combination therapy, add drugs 24h, 48h CCK-8 method to detect cell proliferation, flow cytometry 24h apoptosis Death rate. Results: The results of CCK-8 assay showed that the inhibitory rates of SiHa cells in cervical cancer were significantly higher in other experimental groups than in control group except for cisplatin 1.5μg / ml after treated with β-elemene and cisplatin for 24 hours and 48 hours <0.05), to a certain extent, concentration and time-dependent. Combination therapy, the cell inhibition rate and apoptosis rate was significantly higher than the single drug (P <0.01). Conclusion: β-elemene and cisplatin alone or in combination can inhibit the proliferation of SiHa cells and promote their apoptosis. The effect of β-elemene combined with cisplatin is significantly higher than that of β-elemene and β-elemene And cisplatin synergistic (CDI <1) to promote SiHa cell apoptosis.