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目的:探讨氟伐他汀预处理对脑缺血再灌注损伤大鼠脑组织中Fas、FasL表达的影响。方法:实验大鼠随机分为假手术组(Sham组)、脑缺血再灌注组(I/R组)和氟伐他汀预处理组(Flu组)。线栓法制作大脑中动脉脑缺血再灌注模型,Flu组在模型制备前用氟伐他汀连续灌胃14 d。缺血2 h再灌注24 h后断头取脑,免疫组织化学法和半定量PCR法检测缺血区脑组织中Fas、FasL的表达。结果:通过免疫组织化学法和半定量PCR法检测大鼠缺血区脑组织中Fas和FasL阳性细胞,Sham组仅见少量表达,I/R组表达显著增多(P<0.05),Flu组表达显著减少(P<0.05)。大鼠缺血侧皮质区Fas mRNA和FasL mRNA表达为,Sham组有微弱的表达,I/R组表达显著增高(P<0.05),Flu组表达则显著降低(P<0.05)。结论:氟伐他汀对大鼠脑缺血再灌注损伤发挥保护作用的机制可能与下调Fas、FasL的表达有关。
Objective: To investigate the effect of fluvastatin pretreatment on the expression of Fas and FasL in brain tissue of rats with cerebral ischemia-reperfusion injury. Methods: The rats were randomly divided into Sham group, I / R group and Fluvastatin preconditioning group (Flu group). The model of middle cerebral artery occlusion (MCAO) was established by thread occlusion. Flu group was treated with fluvastatin for 14 days before model preparation. The brains were removed at 2 h after reperfusion for 24 h after ischemia, and the expressions of Fas and FasL were detected by immunohistochemistry and semi-quantitative PCR. Results: Fas and FasL positive cells were detected by immunohistochemistry and semi-quantitative PCR in Sham group, with only a little expression in Sham group, the expression of I / R group was significantly increased (P <0.05), the expression of Flu group was significant Decrease (P <0.05). The expressions of Fas mRNA and FasL mRNA in ischemic cortex of rats were weakly expressed in Sham group, the expression of I / R group was significantly increased (P <0.05), while the expression of Flu group was significantly decreased (P <0.05). Conclusion: The mechanism of fluvastatin on cerebral ischemia-reperfusion injury in rats may be related to down-regulation of Fas and FasL expression.