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本文扼要报导自合成的A链S-磺酸衍生物与天然B链S-磺酸衍生物给合而获得结晶牛胰岛素。前文报导后,我们在A链的合成以及最后半合成产物的纯化方面都作了改进。牛胰岛素A链羧端十二肽Ⅰ,即:N-苄氧羰基缬氨酰-S-苄基半胱氨酰-丝氨酰-亮氨酰-酪氨酰-谷氨酰胺酰-亮氨酰-谷氨酰-天门冬酰胺酰-酪氨酰-S-苄基半胱氨酰-天门冬酰胺,曾从下述两种合成途径获得:(1)已知的三肽酰肼
This paper briefly reports that crystalline bovine insulin is obtained from the synthesis of a synthetic A-chain S-sulfonic acid derivative and a native B-chain S-sulfonic acid derivative. After the previous report, we have made improvements in the synthesis of the A chain and in the purification of the final semisynthetic products. Bovine insulin A chain carboxy terminal dodecapeptide I, namely: N-benzyloxycarbonyl valyl-S-benzyl cysteinyl-seryl-leucyl-tyrosyl-glutaminyl-leucine Acyl-glutamyl-asparaginyl-tyrosyl-S-benzylcysteinyl-asparagine has been obtained from two synthetic routes: (1) the known tripeptide hydrazide