本文报道金属变应原氯化汞和硫酸镍对儿童胸腺细胞和外周血淋巴细胞DNA合成的影响。实验对象为3个月～13岁从未患过湿疹的儿童。在这些儿童进行心脏手术时获得胸腺细胞和外周血淋巴细胞。细胞与金属变应原一起孵育3～6天,终止孵育前6小时加入~3H-胸腺嘧啶,终止培养后测~3H-胸腺嘧啶的脉冲数, This article reports the effects of metal allergens mercuric chloride and nickel sulfate on DNA synthesis in children’s thymocytes and peripheral blood lymphocytes. The subjects were children who had never had eczema for 3 months to 13 years. Thyocytes and peripheral blood lymphocytes were obtained during these children undergoing heart surgery. The cells are incubated with the metal allergen for 3 to 6 days, and ~ 3H-thymidine is added 6 hours before the termination of the incubation, and the number of pulses of ~ 3H-thymidine is measured after termination of the culture,