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目的:探讨在不同肌糖原含量状态下,骨骼肌白细胞介素6(IL-6)基因表达、血清IL-6及可溶性IL-6受体(sIL-6R)的变化规律。方法:雄性SD大鼠88只随机分为空白对照组和运动前低肌糖原组、运动前正常肌糖原组3大组,后两个大组又分别分为安静时、运动30分钟、运动2小时、运动2小时后恢复3小时和运2小时后恢复6小时5个小组。各运动组大鼠先进行糖原消耗运动,在24小时恢复期按分组分别采取不同饮食干预(正常饲料和低糖饲料),再进行下一次定量负荷运动。分别在不同时间点宰杀大鼠,取血清、股四头肌,测血清IL-6与sIL-6R浓度、骨骼肌肌糖原含量与IL-6mRNA。结果:与安静对照组相比,运动组各时间点血清IL-6浓度、血清sIL-6R浓度及骨骼肌IL-6mRNA大多显著升高(P<0.05,P<0.01);运动前低肌糖原组与正常糖原组相比,血清IL-6浓度与骨骼肌IL-6mRNA大多显著升高(P<0.05,P<0.01),但血清sIL-6R浓度无显著性差异(P>0.05)。结论:运动引起血清IL-6和sIL-6R增加;运动前肌糖原含量可能调控运动诱导的骨骼肌IL-6基因的转录,但是对sIL-6R似乎没有调控作用;在特定的运动负荷下,骨骼肌IL-6基因转录的增加可能是在运动后,而不是在运动中。
Objective: To investigate the changes of IL-6 gene expression, serum IL-6 and soluble IL-6 receptor (sIL-6R) in different muscle glycogen contents. Methods: Eighty male Sprague-Dawley rats were randomly divided into 3 groups: control group, pre-exercise low muscle glycogen group and pre-exercise muscle glycogen group. The latter two groups were divided into resting group, exercise group for 30 minutes, 2 hours of exercise, 3 hours of recovery after 2 hours of exercise and 5 hours of recovery of 6 hours after 2 hours of exercise. Rats in each exercise group were given glycogen depletion exercise, and different dietary interventions (normal diet and low-sugar diet) were carried out by group in 24-hour recovery period, and then the next quantitative load exercise. The rats were sacrificed at different time points. Serum and quadriceps femoris were collected for determination of serum IL-6 and sIL-6R concentrations, skeletal muscle glycogen content and IL-6 mRNA. Results: Serum IL-6 concentration, serum sIL-6R concentration and skeletal muscle IL-6 mRNA in exercise group were significantly higher than those in control group at rest (P <0.05, P <0.01) Compared with the normal glycogen group, the serum IL-6 concentration and the skeletal muscle IL-6 mRNA in the original group were significantly increased (P <0.05, P <0.01), but there was no significant difference in serum sIL- . CONCLUSION: Exercise induced increase of serum IL-6 and sIL-6R. Pre-exercise muscle glycogen content may regulate exercise-induced transcription of IL-6 gene in skeletal muscle, but no regulation of sIL-6R appears. Under certain exercise load , Skeletal muscle IL-6 gene transcription may be increased after exercise, rather than during exercise.