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从广东汕尾一鲍鱼养殖厂的患病黑稚鲍中分离筛选到56株菌,并对之进行致病毒力因子(胞外酶及溶血作用)的分析。结果表明,在56株菌中有16株具有较强的分泌胞外蛋白酶、明胶酶、磷脂酶、脂肪酶和淀粉酶的能力,占总菌株数的28.6%;而有溶血现象的仅有6株,占总菌株数的10.7%,且溶血能力不强。其中,8株具有很强的产胞外蛋白酶的能力,3株具有很强的产胞外明胶酶的能力,21株具有产胞外磷脂酶的能力,5株具有产溶血素的能力。此外,同时可分泌4种以上胞外产物的菌株为22株,占39.3%;同时分泌6种胞外产物的菌株为3株。综合考虑每种胞外产物的潜在致病作用以及菌株分泌多种胞外产物的能力,初步确认16株菌为潜在的致病菌。
Fifty-six strains were isolated and screened from diseased juvenile abalone of Shanwei-Abalone breeding factory in Guangdong Province, and their virulence factors (extracellular enzymes and hemolysis) were analyzed. The results showed that 16 out of 56 strains had strong ability to secrete extracellular protease, gelatinase, phospholipase, lipase and amylase, accounting for 28.6% of the total number of strains, while only 6 Strain, accounting for 10.7% of the total number of strains, and hemolytic ability is not strong. Among them, 8 had strong ability to produce extracellular protease, 3 had strong ability to produce extracellular gelatinase, 21 had the ability to produce extracellular phospholipase and 5 had the ability to produce hemolysin. In addition, 22 strains, accounting for 39.3%, were also secretable for more than 4 extracellular products, while 3 strains were also secreting 6 extracellular products. Considering the potential pathogenicity of each extracellular product and the ability of the strain to secrete various extracellular products, 16 strains of bacteria were initially identified as potential pathogenic bacteria.