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目的:观察肠康方对腹泻型肠易激综合征大鼠血浆P物质(SP)和大鼠组织血管活性肠肽(VIP)含量的变化,探讨肠康方对腹泻型肠易激综合征的治疗效果。方法:将60清洁级雄性SD大鼠随机分为正常组、模型组、马来酸曲美布汀组,肠康方低、中、高剂量组。除正常组外,其余各组均采用番泻叶刺激加束缚应激的方法 4周,复制腹泻型肠易激综合征模型,造模成功后开始给药,马来酸曲美布汀组ig马来酸曲美布汀15 mg·kg-1,肠康方低、中、高剂量组分别予以肠康方1.1,2.2,4.4g·kg-1ig给药,正常及模型组ig等体积的生理盐水,每日1次,连续给药3周。给药结束后,进行腹壁撤退反射(AWR)评分,并采集腹主动脉血和盲肠上结肠组织,用酶联免疫分析法测定血浆SP和结肠组织VIP的含量。结果:与正常组比较,模型组大鼠AWR评分,血浆SP水平明显上升(P<0.01),结肠VIP含量显著下降(P<0.05);与模型组比较,肠康方高、中、低剂量组AWR评分明显降低(P<0.01,P<0.05),血浆SP水平明显降低(P<0.05),肠康方高、中剂量组结肠VIP含量明显升高(P<0.01,P<0.05)。结论:肠康方可能通过改善内脏高敏感性、降低血浆中SP的含量和升高结肠黏膜中VIP的含量,治疗腹泻型肠易激综合征。
Objective: To observe the effect of Changkang decoction on the content of plasma substance P (SP) and vasoactive intestinal peptide (VIP) in rats with diarrhea-predominant irritable bowel syndrome and to explore the effect of Changkang decoction on diarrhea-predominant irritable bowel syndrome treatment effect. Methods: Sixty clean male Sprague-Dawley rats were randomly divided into normal group, model group, trimebutine maleate and Changkangfang low, medium and high dose groups. In addition to the normal group, the other groups were treated with senna stimulation and restraint stress for 4 weeks, replication of diarrhea-predominant irritable bowel syndrome model, the successful start after the model administration, trimebutine maleate ig Trimebutine maleate trimebutine 15 mg · kg-1, Changkang Decoction low, medium and high dose groups were given Chang Kang prescriptions 1.1,2.2,4.4g · kg-1ig administration, normal and model group ig equal volume Saline, once daily, for 3 consecutive weeks. At the end of the administration, the abdominal wall reflex (AWR) score, the abdominal aorta blood and the cecal upper colon were collected, and the contents of VIP and VIP in the plasma SP and colon tissues were determined by enzyme-linked immunosorbent assay. Results: Compared with the normal group, the AWR score, plasma SP level and the content of VIP in the model group significantly decreased (P <0.05). Compared with the model group, the levels of high, medium and low The AWR score was significantly lower (P <0.01, P <0.05), and the plasma SP level was significantly lower (P <0.05). The colon VIP content of Changkang decoction group was significantly higher (P <0.01, P <0.05). Conclusion: Changkang decoction may treat diarrhea-predominant irritable bowel syndrome by improving visceral hypersensitivity, decreasing plasma SP content and increasing VIP content in colonic mucosa.