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目的:对银杏多糖进行分离纯化,单糖组成确定并对其抗氧化活性进行测试。方法:用热提醇沉的方法提取多糖,使用Sevag法除蛋白,粗多糖用DEAE-52和DEAE-Sepharose Fast Flow进行色谱分离,精多糖用凝胶排阻色谱法测定相对分子量(Mr),红外光谱检测糖构型,高效离子色谱法测单糖组成,在体外测定其对羟基自由基、DPPH.的消除作用。结果:两个精多糖GBPB-W和GBPB-S的分子量分别为26 300和19 100,均由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖组成,比例分别为(3.48∶8.47∶3.73∶1.76∶1)和(5.34∶5.37∶5.27∶1∶1.68)。GBPB-S具有一定的清除自由基的能力。结论:银杏多糖GBPB-S在体外有直接的抗氧化活性。
OBJECTIVE: To isolate and purify Ginkgo biloba polysaccharide, determine its monosaccharide composition and test its antioxidant activity. Methods: Polysaccharides were extracted by hot extraction and alcohol precipitation, the proteins were removed by Sevag method, and the crude polysaccharides were separated by DEAE-52 and DEAE-Sepharose Fast Flow. The relative molecular weight (Mr) of polysaccharides was determined by gel permeation chromatography. Infrared spectroscopy was used to detect the glycostructure. The composition of monosaccharides was determined by high performance ion chromatography. The elimination of hydroxyl radical and DPPH was determined in vitro. Results: The molecular weights of two polysaccharides GBPB-W and GBPB-S were 26 300 and 19 100, respectively, which were composed of rhamnose, arabinose, galactose, glucose and mannose with the ratios of (3.48:8.47:3.73 : 1.76: 1) and (5.34: 5.37: 5.27: 1: 1.68). GBPB-S has a certain ability to scavenge free radicals. Conclusion: Ginkgo biloba polysaccharide GBPB-S has direct antioxidant activity in vitro.