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目的研究查尔酮类化合物(代号:L2H17,简称L2)对流感病毒感染小鼠的保护作用及引起的病毒性肺炎的治疗作用。方法将小鼠随机分为6组:正常组、模型组、阳性药奥司他韦(达菲)组(20 mg/kg)、L2 20、40、80mg/kg剂量组,适应性喂养72 h,滴鼻(IN)造模,于造模前24 h给药,连续给药6 d观察至14 d,统计各组小鼠的中位生存时间、死亡保护率和生命延长率,观察L2对流感病毒感染小鼠的保护作用;按上法于造模后第3、5天无菌条件取小鼠全肺,计算肺指数及肺指数抑制率,左肺用4%甲醛固定,用于病理组织切片;右肺浸泡于RNAstore,检测肺组织病毒载量;小鼠摘眼球取血,采用双抗体夹心ELISA法检测小鼠血清中炎症细胞因子IL-6、TNF-α含量,观察L2对流感病毒感染引起的病毒性肺炎的治疗作用。结果与模型组相比,L2 80 mg/kg剂量组死亡率下降50%,中位生存时间、死亡保护率、生命延长率均有所提高;造模后第3、5天,L2 80 mg/kg剂量组肺指数降低,病理形态也有所改善;小鼠肺组织病毒载量降低(P<0.05);炎症因子IL-6含量较模型组有不同程度的降低。结论L2对流感病毒感染小鼠具有一定的保护作用,能减轻流感病毒所致肺炎的病变程度。
Objective To study the protective effect of chalcones (code: L2H17, abbreviated as L2) on mice infected with influenza virus and the therapeutic effect of viral pneumonia. Methods The mice were randomly divided into 6 groups: normal group, model group, oseltamivir (20 mg / kg), L2, 20, 40 and 80 mg / , Intranasal (IN) model, administered 24 h before model making and continuous dosing for 6 d until 14 d. The median survival time, the rate of death protection and the rate of life prolongation were calculated. Influenza virus infection in mice protective effect; according to the method in the first 3,5 days after modeling aseptic conditions, mice were taken from the whole lung to calculate the lung index and lung index inhibition rate, left lung fixed with 4% formaldehyde for pathology Tissue sections were obtained. The right lungs were soaked in RNAstore to detect the lung tissue viral load. The mice were eyedrops and the blood was collected. The levels of inflammatory cytokines IL-6 and TNF-α were detected by double antibody sandwich ELISA. Viral infection caused by the treatment of viral pneumonia. Results Compared with the model group, the mortality rate of L280 mg / kg group decreased by 50%, and the median survival time, the rate of death protection and the rate of life prolongation increased. On the 3rd and 5th day after modeling, L2 80 mg / kg dose group decreased lung index, pathological morphology also improved; mouse lung tissue viral load decreased (P <0.05); inflammatory cytokines IL-6 levels were lower than the model group. Conclusion L2 has a protective effect on mice infected with influenza virus and can reduce the severity of pneumonia caused by influenza virus.