抗癌效应细胞(NK、LAK、CTL、Mφ)细胞毒效应、细胞因子和肿瘤化疗药敏检测目前多采用同位素法,但存在着使用同位素而引起的弊端。寻找一种简便,敏感及安全的检测手段已成为当务之急。我们将MTT比色法用于抗癌效应细胞功能、细胞因子及化疗药敏测定,并与同位素方法进行了对比,结果表明两种方法具有良好的相关性,MTT法与后者相比更为简便、经济、安全和实用。 Cytotoxicity of anti-cancer effector cells (NK, LAK, CTL, Mφ), cytokines, and chemotherapeutic sensitivity of tumors are currently used for isotope methods, but there are drawbacks caused by the use of isotopes. Finding a simple, sensitive and safe detection method has become an urgent task. We used MTT colorimetric assay for anti-cancer effector cell functions, cytokines, and chemotherapeutic susceptibility assays, and compared them with isotope methods. The results show that the two methods have good correlations, and MTT assays are more advanced than the latter. Simple, economical, safe and practical.