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为探讨外周血嗜酸粒细胞在IL 3、TNF α、IFN γ、佛波酯 (PMA )等刺激剂诱导下释放超氧离子 (O2 )的能力 ,我们采用超氧化物歧化酶 (SOD ) 抑制细胞色素C还原法检测嗜酸粒细胞产生O2 的量 ,结果发现 :(1)一定浓度的IL 3、PMA、TNF α能诱导嗜酸粒细胞释放大量的O2 ,与空白对照有显著性差异 ,而且以PMA的诱导能力最强 ;(2 )IFN γ的刺激量高达 10 0 0u/ml时 ,也不能有效促进嗜酸粒细胞释放O2 ,与空白对照无显著性差异 ;(3)健康献血者与哮喘等变应性疾病患者的外周血嗜酸粒细胞在相同的刺激剂诱导下释放的O2 量无显著性差异。以上结果说明 :IL 3、PMA和TNF α是嗜酸粒细胞产生O2 的强有力的诱导剂 ,而IFN γ则无此作用 ,从而提示应用IL 3、PMA和TNF α的拮抗剂有可能抑制嗜酸粒细胞释放O2 。
To investigate the ability of peripheral blood eosinophils to release superoxide anion (O2) induced by IL 3, TNFα, IFNγ, phorbol myristate (PMA) and other stimulants, we used superoxide dismutase (SOD) Cytochrome C reduction method to detect eosinophil production of O2, the results showed that: (1) a certain concentration of IL 3, PMA, TNF α can induce large numbers of eosinophils release of O2, and the blank control were significantly different, (2) IFNγ stimulated up to 10 0u / ml, it can not effectively promote eosinophil release of O2, no significant difference with the blank control; (3) healthy blood donors There were no significant differences in the amount of O2 released by the peripheral blood eosinophils of patients with allergic diseases such as asthma under the same stimulus. The above results indicate that IL 3, PMA and TNFα are potent inducers of eosinophil production of O2, whereas IFNγ has no effect, suggesting that the use of antagonists of IL 3, PMA and TNFα may inhibit Acid granulocytes release O2.