论文部分内容阅读
1997年5～10月,对全县27家医院进行消毒效果监测,从1029个空气采样标本中,检出具有溶血力的菌落2091个.为了解此类细菌在医院空气中的分布及耐药情况,对其进行了菌株鉴定及耐药谱分析.1 材料与方法按卫生部《消毒技术规范》中空气采样及检查方法,采用空气平皿暴露法.凡在血平皿上产生溶血的菌落,进行纯培养后供鉴定用.将纯化后溶血的菌落,先行革兰氏染色,再根据染色性质、菌体形态,按文献进行综合判定种或属.耐甲氧西林葡萄球菌的鉴定按文献方法进行.药敏试验用K—B纸片法,按NC-CLS标准判定结果.质控菌株为金葡菌(ATC-C25923)、大肠杆菌(ATCC25922)、绿脓杆菌(ATC-C27853).
From May to October 1997, 27 hospitals across the county were monitored for disinfection effects. Among 1,029 air sampling samples, 2091 colonies with hemolytic power were detected. To understand the distribution and resistance of these bacteria in hospital air The situation, the strain identification and analysis of drug resistance spectrum. 1 Materials and methods According to the air sampling and inspection methods in the “Disinfection Technical Specifications” of the Ministry of Health, the air-plate exposure method was used. Wherever hemolytic colonies are produced on the blood plate, carry out After the pure culture for identification, the purified hemolytic colonies were first stained with Gram, and then the species or genus was comprehensively judged according to the staining characteristics and the morphology of the bacteria. The identification of methicillin-resistant Staphylococcus was performed according to the literature method. Drug susceptibility test using K-B paper method, according to NC-CLS standard results. Quality control strains are S. aureus (ATC-C25923), E. coli (ATCC25922), Pseudomonas aeruginosa (ATC-C27853).