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目的观察脱氧胆酸钠体外对致病株白色念珠菌菌丝相胞外磷脂酶的抑制作用。方法在37℃下,用蛋黄平板法培养并测量菌株产生沉淀圈的大小,用沉淀圈比值(PZ值)比较磷脂酶活力的变化。结果对照组与脱氧胆酸钠不同浓度组(依次为0.1%、0.2%、0.3%和0.4%)的PZ值分别为0.389±0.049、0.778±0.098、0.882±0.077、0.912±0.049和0.888±0.093;脱氧胆酸钠各浓度组与对照组比较差异均有非常显著性(P<0.001)。脱氧胆酸钠不同浓度组间PZ值比较,0.1%和0.2%、0.1%和0.3%、0.1%和0.4%组间差异有显著性(P<0.05)。结论脱氧胆酸钠体外对白色念珠菌菌丝相胞外磷脂酶具有抑制作用。
Objective To observe the inhibitory effect of sodium deoxycholate on the extracellular phospholipase of Candida albicans in vitro. Methods The size of sedimentation circle was measured by egg yolk plate method at 37 ℃. The change of phospholipase activity was compared with the sedimentation circle ratio (PZ value). Results The PZ values of the control group and sodium deoxycholate at different concentrations (0.1%, 0.2%, 0.3% and 0.4%, respectively) were 0.389 ± 0.049, 0.778 ± 0.098, 0.882 ± 0.077, 0.912 ± 0.049 and 0.888 ± 0.093 ; Sodium deoxycholate concentration in each group compared with the control group were significantly different (P <0.001). PZ values of different concentrations of sodium deoxycholate group were significantly different between 0.1% and 0.2%, 0.1% and 0.3%, 0.1% and 0.4% groups (P <0.05). Conclusion Sodium deoxycholate has inhibitory activity on extracellular phospholipase of Candida albicans in vitro.