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目的 研究血红素氧化酶 1(HO 1)在大鼠同种异体心脏移植模型中的组织表达以及环孢素A (CsA)对该表达的影响。方法 心脏移植大鼠分为Ⅰ组 (对照组 )、Ⅱ组 (CsA 7 5mg/KgB W .)和Ⅲ组 (CsA 15mg/KgB .W .)。依据国际心肺移植组织 (ISHLT) 1990年心脏急性排异反应分级标准进行排异反应分析 ;免疫组化方法检测HO 1的表达。结果 心脏移植后 ,供者心脏和主动脉标本中 ,1和 3天时出现轻度炎性浸润 ,排异反应为 1A或 1B级 ;11天和 12天时出现弥漫性炎性浸润和心肌坏死 ,排斥级别达 3B或 4级。CsA治疗可使心脏移植排异反应降低 1～ 2 5级。移植后HO 1广泛表达于受体各类组织中 ,其中尤其以肝、肾、脾等器官表达增强比较明显 ,供心心肌细胞、血管内皮细胞和部分浸润淋巴细胞的HO 1表达均显著升高 ,CsA在一定时间内可明显增强供者心脏及主动脉的HO 1表达 ,减低移植排斥反应 ,从而延长移植心脏存活时间。结论 HO 1在心脏移植后受者体内表达广泛增强 ,CsA治疗可增强HO 1表达 ,这可能与其减弱移植排斥反应机制相关。
Objective To investigate the tissue expression of heme oxygenase 1 (HO 1) in rat heart allografts and the effect of cyclosporin A (CsA) on the expression. Methods Heart transplantation rats were divided into group Ⅰ (control group), group Ⅱ (CsA 7 5mg / KgB W.) And group Ⅲ (CsA 15mg / KgB .W.). According to the International Heart Lung Transplantation Organization (ISHLT) 1990 acute rejection classification criteria for rejection analysis; immunohistochemical detection of HO 1 expression. Results After heart transplantation, donor hearts and aortic specimens showed mild inflammatory infiltration at days 1 and 3, with rejection rates of 1A or 1B; diffuse inflammatory infiltration and myocardial necrosis occurred on days 11 and 12, with exclusion Level 3B or 4. CsA treatment can reduce the rejection rate of heart transplantation 1 ~ 2 5 level. After transplantation, HO 1 was widely expressed in various tissues of the recipient, especially in organs such as liver, kidney and spleen. The expression of HO 1 in cardiac muscle cells, vascular endothelial cells and partially infiltrating lymphocytes were significantly increased , CsA can significantly enhance the HO 1 expression in the donor heart and aorta for a certain period of time, reduce the graft rejection and prolong the survival time of the transplanted heart. Conclusions HO 1 is widely expressed in recipients after heart transplantation. CsA treatment may enhance the expression of HO 1, which may be related to its mechanism of attenuating allograft rejection.