目的 :研究切应力作用下与血管平滑肌细胞联合培养的内皮细胞的抗凝血功能。 方法 :应用流动腔系统 ,对与平滑肌细胞联合培养的内皮细胞施加生理大小的切应力 ,以放免分析法检测内皮细胞分泌 PGI2 的变化。 结果 :PGI2 峰值释放速率及稳定释放速率随切应力的增大而增高 ,峰值释放速率的衰减常数 (t1 / 2 )随切应力的增大而减小。 结论 :切应力作用下 ,与平滑肌细胞联合培养的内皮细胞的抗凝血功能增强 Objective: To study the anticoagulant function of endothelial cells co-cultured with vascular smooth muscle cells under shear stress. METHODS: Physiological shear stress was applied to endothelial cells co-cultured with smooth muscle cells by flow cell system, and the changes of PGI2 secretion by endothelial cells were detected by radioimmunoassay. Results: The peak release rate and the steady release rate of PGI2 increased with the increase of shear stress. The decay constant of peak release rate (t1 / 2) decreased with the increase of shear stress. CONCLUSION: The anticoagulant function of endothelial cells cultured in combination with smooth muscle cells is enhanced under shear stress