论文部分内容阅读
目的分析胰腺实性-假乳头状肿瘤(SPN)的临床病理特征。方法 HE染色观察7例SPN的形态学特征,免疫组化检测多种蛋白的表达。结果 7例均为女性,发病年龄15～70岁,平均36.7岁。镜下瘤组织表现为特征性的实性片状区及假乳头状结构SPN,其中1例发现包膜外侵犯及血管浸润。所有病例随访18～36个月,均未见复发、转移。免疫组化示7例肿瘤组织vimentin、NSE、PR和α-1-AT均弥漫(+);6例CD56、CD10弥漫(+);所有病例ER和CK均(-)。相对于正常胰腺组织内E-cadherin和β-catenin的胞膜阳性表达,7例瘤细胞E-cadherin均(-),而β-catenin在胞质和胞核内强(+):5例瘤细胞cyclin D1不同程度(+)(阳性率18%～90%)。结论 SPN具有特征性的组织学结构,vimentin、CD56、CD10、PR、α-1-AT和NSE普遍(+);β-catenin核/质强(+)、E-cadherin(-)及cyclin D1强(+)也是SPN的重要病理学特征。
Objective To analyze the clinicopathological features of solid pancreatic pseudopapillary tumor (SPN). Methods The morphological features of 7 cases of SPN were observed by HE staining. The expressions of various proteins were detected by immunohistochemistry. Results 7 cases were female, the onset age of 15 to 70 years, an average of 36.7 years old. Microscopic tumor tissue showed characteristic solid patchy area and pseudopapillary structure SPN, including 1 case of extracapsular invasion and vascular invasion. All cases were followed up 18 to 36 months, no recurrence, metastasis. Immunohistochemistry showed that vimentin, NSE, PR and α-1-AT were all diffuse (+) in 7 tumor tissues; 6 were CD56 and CD10 diffusely (+); ER and CK were all (-) in all cases. The positive expression of E-cadherin and β-catenin in normal pancreatic tissue was found in all seven tumor cells, but β-catenin was strongly (+) in cytoplasm and nucleus: 5 tumors Cell cyclin D1 varying degrees (+) (positive rate of 18% to 90%). Conclusions SPN has the characteristic histological structure, vimentin, CD56, CD10, PR, α-1-AT and NSE are generally (+); β-catenin nuclear / Strong (+) is also an important pathological feature of SPN.