论文部分内容阅读
目的:观察有氧运动对糖尿病肾损伤大鼠肾功能保护作用,并探讨有氧运动改善糖尿病大鼠肾病的作用机制。方法:建立糖尿病大鼠肾损伤模型和有氧运动模型。比较大鼠血糖、肾脏肥大指数(肾重/体重)、尿素氮(urea nitrogen,BUN)、血肌酐(Cr)2、4 h尿蛋白(Urine protein 24 hours;UP24)等肾功能相关指标,比较各组血清超氧化物歧化酶(Superoxide dismutas,SOD)活性、脂质过氧化代谢产物丙二醛(malondialdehyde,MDA)含量、肾组织醛糖还原酶(aldose reductase,AR)、转化生长因子β1(Transforming growth factor-β1,TGF-β1)和Smad表达的改变。结果:有氧运动组与模型对照组相比,能明显降低血糖、有效控制体重下降、明显改善肾脏肥大指数、BUN、Cr、UP24、MDA含量显著降低(P<0.05),血清SOD活性增强,肾脏AR mR-NA、TGF-β1 mRNA和Smad蛋白表达水平下调(P<0.05)。结论:有氧运动对糖尿病肾损伤大鼠可能通过提高机体抗氧化能力、减少肾脏AR基因表达以抑制多元醇通路的激活、减少TGF-β1 mRNA和Smad蛋白表达以抑制TGF-β1/Smad信号通路等方面发挥其肾功能的保护作用。
Objective: To observe the protective effect of aerobic exercise on renal function in rats with diabetic nephropathy and to explore the mechanism of aerobic exercise on improving nephropathy in diabetic rats. Methods: To establish a model of renal injury and aerobic exercise in diabetic rats. The indexes of renal function such as blood glucose, renal hypertrophy index (renal weight / body weight), urea nitrogen (BUN) and urinary protein 24 hours (U24) were compared. The activities of serum superoxide dismutase (SOD), malondialdehyde (MDA), aldose reductase (AR), transforming growth factor β1 Transforming growth factor-β1, TGF-β1) and Smad expression. Results: Compared with the model control group, the aerobic exercise group could significantly lower the blood glucose, effectively control the weight loss, and significantly improve the renal hypertrophy index. The levels of BUN, Cr, UP24 and MDA were significantly decreased (P <0.05) The renal AR mR-NA, TGF-β1 mRNA and Smad protein levels were down-regulated (P <0.05). CONCLUSION: Aerobic exercise can inhibit the expression of TGF-β1 / Smad signal pathway in diabetic rats with renal injury by increasing the body’s antioxidant capacity, decreasing renal AR gene expression, inhibiting the activation of polyol channels, and decreasing the expression of TGF-β1 mRNA and Smad protein And so on to play its role in the protection of renal function.