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应用免疫组化及原位杂交方法,对良恶性鼻咽组织中C-erbB-2表达及增殖细胞核抗原(PCNA)进行检测。结果显示:鼻咽癌(NPC)组织中C-erbB-2蛋白和mRNA阳性率分别为87.8%和84.0%,二者同时表达为84.0%;癌旁粘膜中二者阳性率分别为74.6%和76.5%,同时表达为82.4%。泡状核细胞癌PCNASⅡ值明显高于低分化鳞癌(P<0.01)。癌组织中C-erbB-2蛋白阳性病例PCNASⅡ值高于阴性病例(P<0.05)。提示:C-erbB-2癌基因的过度表达以及由此导致的细胞异常增殖与NPC发生发展有关;C-erbB-2和PCNA联合检测,对判断肿瘤恶性程度及预测NPC放疗效果有一定意义
Immunohistochemistry and in situ hybridization were used to detect the expression of C-erbB-2 and the proliferating cell nuclear antigen (PCNA) in benign and malignant nasopharyngeal tissues. The results showed that the positive rates of C-erbB-2 protein and mRNA in nasopharyngeal carcinoma (NPC) were 87.8% and 84.0%, respectively. The positive rates of C-erbB-2 protein and mRNA were 84.0% Rates were 74.6% and 76.5%, respectively, while expressing 82.4%. PCNAS Ⅱ was significantly higher in poorly differentiated squamous cell carcinoma (P <0.01). The positive value of PCNAS in C-erbB-2 protein positive cases was higher than that in negative cases (P <0.05). It is suggested that the overexpression of C-erbB-2 oncogene and the consequent cell proliferation are related to the occurrence and development of NPC. The combined detection of C-erbB-2 and PCNA has some significance in judging the degree of malignancy and predicting the radiotherapy effect of NPC