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应用免疫组化及原位杂交方法，对良恶性鼻咽组织中Ｃ－ｅｒｂＢ－２表达及增殖细胞核抗原（ＰＣＮＡ）进行检测。结果显示：鼻咽癌（ＮＰＣ）组织中Ｃ－ｅｒｂＢ－２蛋白和ｍＲＮＡ阳性率分别为８７．８％和８４．０％，二者同时表达为８４．０％；癌旁粘膜中二者阳性率分别为７４．６％和７６．５％，同时表达为８２．４％。泡状核细胞癌ＰＣＮＡＳⅡ值明显高于低分化鳞癌（Ｐ＜０．０１）。癌组织中Ｃ－ｅｒｂＢ－２蛋白阳性病例ＰＣＮＡＳⅡ值高于阴性病例（Ｐ＜０．０５）。提示：Ｃ－ｅｒｂＢ－２癌基因的过度表达以及由此导致的细胞异常增殖与ＮＰＣ发生发展有关；Ｃ－ｅｒｂＢ－２和ＰＣＮＡ联合检测，对判断肿瘤恶性程度及预测ＮＰＣ放疗效果有一定意义
Immunohistochemistry and in situ hybridization were used to detect the expression of C-erbB-2 and the proliferating cell nuclear antigen (PCNA) in benign and malignant nasopharyngeal tissues. The results showed that the positive rates of C-erbB-2 protein and mRNA in nasopharyngeal carcinoma (NPC) were 87.8% and 84.0%, respectively. The positive rates of C-erbB-2 protein and mRNA were 84.0% Rates were 74.6% and 76.5%, respectively, while expressing 82.4%. PCNAS Ⅱ was significantly higher in poorly differentiated squamous cell carcinoma (P <0.01). The positive value of PCNAS in C-erbB-2 protein positive cases was higher than that in negative cases (P <0.05). It is suggested that the overexpression of C-erbB-2 oncogene and the consequent cell proliferation are related to the occurrence and development of NPC. The combined detection of C-erbB-2 and PCNA has some significance in judging the degree of malignancy and predicting the radiotherapy effect of NPC