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用新生 Wistar大鼠进行脊髓神经元培养 ,研究微管相关蛋白 - 5与其单克隆抗体结合后的分布情况。使用微管解聚药 nocodazole处理神经元 ,应用免疫组织化学染色来观察微管相关蛋白 - 5的改变。另一组神经元使用 nocodazole处理后再用 PMA处理 ,观察微管相关蛋白 - 5及神经元的改变。结果表明 ,微管相关蛋白 - 5在胞浆及突起中均有分布 ,并随着培养天数的递增而递减。使用 nocodazole后神经元中微管相关蛋白 - 5的分布及数量明显减少。PMA处理神经元后尽管使微管相关蛋白 - 5的正常结构被破坏 ,而神经元的伸展却不受影响
Spinal cord neurons were cultured with newborn Wistar rats to study the distribution of microtubule-associated protein-5 after binding with its monoclonal antibody. Neurons were treated with microtubule depolymerization nocodazole, and the changes of microtubule-associated protein-5 were observed by immunohistochemical staining. Another group of neurons were treated with nocodazole and then treated with PMA to observe changes in microtubule-associated protein-5 and neurons. The results showed that microtubule-associated protein-5 was distributed in both cytoplasm and protuberance, and decreased with the increasing number of days. After administration of nocodazole, the distribution and number of microtubule - associated protein - 5 in neurons decreased significantly. After PMA treatment of neurons, although the normal structure of microtubule-associated protein-5 was disrupted, the extension of neurons was not affected