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采用荧光定量PCR分析表明Zma-miR169i受干旱胁迫和脱落酸诱导表达。以玉米叶片基因组DNA为材料,根据预测的Zma-miR169i启动子序列设计引物,采用PCR方法克隆得到Zma-miR169i上游一段长度为1 827 bp的启动子序列。利用启动子预测网站Plant CARE分析发现,Zma-miR169i启动子序列具有CAAT-box、TATA-box基本的顺式作用元件和一些参与植物激素和非生物胁迫应答的相关顺式作用元件。研究结果为进一步分析Zma-miR169i的功能奠定了基础。
Quantitative real-time PCR analysis showed that Zma-miR169i was induced by drought stress and abscisic acid. Primers were designed based on the predicted Zma-miR169i promoter using the genomic DNA of maize leaves. A 1827-bp promoter sequence upstream of Zma-miR169i was cloned by PCR. Based on the Plant CARE analysis of the promoter prediction site, the Zma-miR169i promoter sequence has the CAAT-box, the basic cis-acting element of TATA-box and some cis-acting elements involved in plant hormone and abiotic stress responses. The results laid the foundation for further analysis of the function of Zma-miR169i.