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目的:探讨血浆转化生长因子(TGFβ1)和纤溶酶原激活物抑制因子1(PAI1)与2型糖尿病患者动脉粥样硬化的相关性。方法:利用彩色多普勒超声技术检测62例2型糖尿病患者颈动脉内中膜厚度(IMT)及粥样斑块的形成,将其分为IMT增厚组、斑块形成组和单纯糖尿病组。应用双抗体夹心ELISA法检测其TGFβ1和PAI1血浆含量,同时以28例健康人作为对照组。结果:①2型糖尿病各组血浆TGFβ1和PAI1含量均较对照组显著升高(均P<0.01);IMT增厚组血浆TGFβ1、PAI1含量较单纯糖尿病组显著升高(P<0.05),斑块形成组血浆TGFβ1含量较IMT增厚组显著降低(P<0.05),PAI1含量较IMT组显著升高(P<0.05)。②2型糖尿病患者血浆TGFβ1与PAI1呈正相关(r=0.24,P<0.05);PAI1与IMT呈正相关(r=0.396,P<0.01)。结论:2型糖尿病患者血浆TGFβ1和PAI1的继发性改变在糖尿病动脉粥样硬化发生发展中起重要作用。
Objective: To investigate the correlation between plasma transforming growth factor (TGFβ1) and plasminogen activator inhibitor 1 (PAI1) and atherosclerosis in type 2 diabetic patients. Methods: The carotid intima-media thickness (IMT) and plaque formation in 62 patients with type 2 diabetes mellitus were detected by color Doppler ultrasonography. They were divided into IMT thickening group, plaque formation group and simple diabetic group . The plasma levels of TGFβ1 and PAI1 were detected by double antibody sandwich ELISA. At the same time, 28 healthy volunteers were used as the control group. Results: ① The levels of TGFβ1 and PAI1 in plasma in T2DM group were significantly higher than those in control group (all P <0.01). The levels of TGFβ1 and PAI1 in IMT group were significantly higher than those in simple diabetic group (P <0.05) The level of plasma TGFβ1 in the formation group was significantly lower than that in the IMT group (P <0.05), and the content of PAI1 in the formation group was significantly higher than that in the IMT group (P <0.05). ② There was a positive correlation between plasma TGFβ1 and PAI1 in type 2 diabetic patients (r = 0.24, P <0.05). There was a positive correlation between PAI1 and IMT (r = 0.396, P <0.01). Conclusion: The secondary changes of plasma TGFβ1 and PAI1 in type 2 diabetic patients play an important role in the development of atherosclerosis in diabetic patients.