应用上皮电压钳技术及Ｕｓｓｉｎｇ上皮组织灌流系统，观察蛙直肠上皮的短路化电流（Ｉｓｃ）和活性物质对蛙直肠上皮短路化电流的作用。实验结果表明氨氯吡咪（Ａｍｉｌｏｒｉｄｅ）能有效地抑制蛙直肠上皮的短路化电流；在Ａｍｉｌｏｒｉｄｅ存在的情况下，β肾上腺素能化合物可加强蛙直肠上皮的短路化电流；提示蛙直肠上皮短路化电流依赖Ｎａ＋的内向转运；Ｎａ＋内向转运过程受β肾上腺素能化合物的调控，但是这种调控机制还需要进一步的研究。 Epithelial voltage clamp technique and Ussing epithelial tissue perfusion system were used to observe the effects of short-circuit current (Isc) and active substances on the short-circuit current of frog rectal epithelium. The experimental results show that amiloride can effectively inhibit the short circuit current in the rectum of the breast; in the presence of Amiloride, β-adrenergic compounds enhance the short circuit current in the rectum of the rectum; The current depends on Na + inward transport; Na + inward transport is regulated by β-adrenergic compounds, but this regulatory mechanism needs further study.