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目的 研究沈阳地区家庭聚集性感染乙型肝炎病毒的家庭HBV前C区 1896位G→A基因突变率及其临床意义。方法 采用PCR RFLP法检测HBV前C区 1896位G→A基因突变。结果 患者及其家庭成员HBV前C区 1896位G→A基因突变发生率分别为 5 6 .3%和 4 0 .5 % ,明显高于患者配偶 2 5 0 %的突变发生率 ,且配偶中抗 HBs阳性率为 2 6 .3%。同时 ,这种突变在慢性乙型肝炎患者中的发生率为 5 2 .4 % ,在HBV携带者中的发生率为 4 4 .4 % ,在慢性重型肝炎患者中的发生率仅为 2 0 0 %。结论 HBV前C区 1896位G→A基因突变的发生 ,可能与HBV的持续感染有关。
Objective To investigate the mutation rate of G → A gene in pre-HBV C region of hepatitis B virus (HBV) in families with agglomeration in Shenyang and its clinical significance. Methods The mutation of 1896 G → A gene in pre-HBV C region was detected by PCR RFLP. Results The incidence of G → A mutation at 1896 locus in HBV pre-C region was 56.3% and 40.5% respectively in patients and their family members, which was significantly higher than that of the spouse The positive rate of anti-HBs was 26.3%. At the same time, the mutation rate was 5.24% in patients with chronic hepatitis B, 44.4% in HBV carriers and 20% in patients with chronic severe hepatitis 0%. Conclusion The occurrence of G → A gene mutation at 1896 in pre-HBV C region may be related to the persistent infection of HBV.