目的检测TRAIL、c-FLIP在结直肠癌中的表达情况,探讨二者在大肠肿瘤的浸润转移及凋亡调控机制的可能相互关系和作用。方法选取新鲜结直肠癌、配对的癌旁正常组织各85例及转移淋巴结51例,运用Max Vision免疫组化和Western blot蛋白定量检测TRAIL、c-FLIP在各组间的表达,所得结果进行统计学分析。结果 (1)TRAIL在结直肠癌组织中的定位及表达位于细胞膜及细胞浆,而c-FLIP主要位于细胞浆。(2)TRAIL在癌组、转移淋巴结(LNM)组与癌旁正常组的均广泛表达,三者比较差异无统计学意义(P>0.05);癌组中,未/低分化阳性表达与中高分化比较、无LNM组与LNM组比较差异无统计学意义(P>0.05);(3)c-FLIP在癌组阳性表达明显高于正常组(且无一例强染色);转移淋巴结组阳性表达亦明显高于正常组,其强阳性表达88.2%(45/51),与癌组比较差异无统计学意义(P>0.05)。其癌组中未发生淋巴转移阳性表达88.2%(30/34)、淋巴转移100%(51/51)、未/低分化和中高分化分别为96.8%(30/31)、94.4%(51/54),81例阳性表达中强阳性74%(60/81);未/低分化腺癌与中高分化组表达比较差异无统计学意义(P>0.05)。(4)Western blot蛋白定量检测发现,对于TRAIL,呈现出c-FLIP蛋白表达越高,其表达也反应性增高的趋势,TRAIL蛋白的表达与c-FLIP的表达呈相互拮抗性正相关。结论 (1)c-FLIP在结直肠癌组织和转移淋巴结中存在过高表达,在正常组织低表达或不表达,而TRAIL在癌组织和转移淋巴结组织也存在相互拮抗性增高趋势。(2)高表达的c-FLIP可能与TRAIL死亡受体凋亡途径相互作用,对大肠癌的发生进展起到一定的作用。 Objective To detect the expression of TRAIL and c-FLIP in colorectal cancer, and to explore the possible relationship between the two in the invasion and metastasis of colorectal cancer and the mechanism of apoptosis. Methods Totally 85 cases of fresh colorectal cancer, matched paracancerous normal tissues and 51 cases of metastatic lymph nodes were selected. The expressions of TRAIL and c-FLIP in each group were detected by Max Vision immunohistochemistry and Western blot, and the results were statistically analyzed Analysis. Results (1) The localization and expression of TRAIL in colorectal cancer tissues were located in the cell membrane and cytoplasm, while the c-FLIP mainly located in the cytoplasm. (2) The expression of TRAIL in cancer group, LNM group and normal para-tumorous group were all extensively expressed, with no significant difference between the three groups (P> 0.05). The expression of TRAIL / (3) The positive expression of c-FLIP in the cancer group was significantly higher than that in the normal group (with no case of strong staining); the expression of c-FLIP in the metastatic lymph node group was significantly higher than that in the LNM group (P> 0.05) Also significantly higher than the normal group, the strong positive expression of 88.2% (45/51), compared with the cancer group was no significant difference (P> 0.05). The positive rate of lymphatic metastasis was 88.2% (30/34) in lymphatic metastasis and 100% (51/51) in lymphatic metastasis. The rates of non-well differentiated and well differentiated were 96.8% (30/31) and 94.4% (51 / 54). The positive expression in 81 cases was strongly positive 74% (60/81). There was no significant difference in the expression between non-poorly differentiated adenocarcinoma and well-differentiated adenocarcinoma (P> 0.05). (4) Quantitative detection of Western blot showed that the higher the expression of c-FLIP protein and the higher the reactivity of TRAIL, the higher the TRAIL expression was and the higher the expression of c-FLIP was. Conclusions (1) c-FLIP is overexpressed in colorectal cancer tissues and metastatic lymph nodes, which is low or not expressed in normal tissues. There is also an increasing tendency of TRAIL in cancerous tissues and metastatic lymph nodes. (2) The high expression of c-FLIP may interact with the apoptotic pathway of TRAIL death receptor, which may play a role in the development of colorectal cancer.