[Objective]The aim was to provide a scientific basis for the introduction and cultivation of Fructus cannabis by identifying the resistance of the germplasms from different habitats. [Method] The drought, cold and waterlogging tolerances of 21 representative Fructus cannabis germplasms resources from different parts of China were determined by applying pot culture to control the water and temperature. The performance of flea beetle resistance of Fructus cannabis germplasms was determined by surveying and observing the germplasms cultivated in field. The drought resistance, waterlogging tolerance, cold resistance and flea beetle resistance of 21 Fructus cannabis germplasms resources were comprehensively evaluated by calculating the resistance recovery index (RI) and population density of pests with the membership function, and the synthetical resistance was evaluated by weight grade method. [Result] The comprehensive resistance score of 21 Fructus cannabis was between 49.85 and 84.1. The comprehensive resistance of Fructus cannabis originated in Hohhot (Inner Mongolia), Jingbian (Shaanxi Province) and Lu feng (Yunnan Province) ranked the first, second and third respectively, and that originated in Xifeng, Guizhou Province ranked the last. [Conclusion] The germplasm resistance of Fructus cannabis from different habitats varies, for which the introduction and cultivation of Fructus cannabis should base on the local climatic conditions. [Objective] The aim was to provide a scientific basis for the introduction and cultivation of Fructus cannabis by identifying the resistance of the germplasms from different habitats. [Method] The drought, cold and waterlogging tolerances of 21 representative Fructus cannabis germplasms resources from different parts of China were determined by applying pot culture to control the water and temperature. The performance of flea beetle resistance of Fructus cannabis germplasms was determined by surveying and observing the germplasms cultivated in field. The drought resistance, waterlogging tolerance, cold resistance and flea beetle resistance of 21 Fructus cannabis germplasms resources were comprehensively evaluated by calculating the resistance recovery index (RI) and population density of pests with the membership function, and the synthetical resistance was evaluated by weight grade method. [Result] The comprehensive resistance score of 21 Fructus cannabis was between 49.85 and 84.1. Th e comprehensive resistance of Fructus cannabis originated in Hohhot (Inner Mongolia), Jingbian (Shaanxi Province) and Lu feng (Yunnan Province) ranked the first, second and third respectively, and that originated in Xifeng, Guizhou Province ranked the last. The germplasm resistance of Fructus cannabis from different habitats, for which the introduction and cultivation of Fructus cannabis should base on the local climatic conditions.