In order to investigate effects of topdressing on grain yield and N use efficiency of mechanically-transplanted hybrid rice, this experiment was conducted with 2-line hybrid rice cultivars, Quanliangyou 1 and Quanliangyou 681 with 3 application levels of N fertilizer, N_0, N_1, N_2, respectively. The results showed that there were no significant differences in grain yields of the 2 varieties between treatments N_1 and N_2. It was indicated that N is not the factor which could limit grain yield within limited application levels of N fertilizer. High N fertilizer treatment could contribute to the formation of effective panicles and spikelets per panicle, but it could also lead to the decline in grain filling rate. It was suggested that lowering the amount of Nfertilizer could facilitate improvement of N-use efficiency. In order to investigate effects of top dressing on grain yield and N use efficiency of mechanically-transplanted hybrid rice, this experiment was conducted with 2-line hybrid rice cultivars, Quanliangyou 1 and Quanliangyou 681 with 3 application levels of N fertilizer, N_0, N_1, N 2, respectively. The results showed that there were no significant differences in grain yields of the 2 varieties between treatments N_1 and N_2. It was indicated that N is not the factor which could limit grain yield within limited application levels of N fertilizer. High N fertilizer treatment could contribute to the formation of effective panicles and spikelets per panicle, but it could also lead to the decline in grain filling rate. It was suggested that lowering the amount of Nfertilizer could facilitate improvement of N-use efficiency.