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NAC转录因子在植物发育和逆境应答中具有重要的作用。本研究从大豆中克隆了1个NAC基因GmNAC115。该基因c DNA全长1 383 bp,开放阅读框长912 bp,编码303个氨基酸,预测分子量约为34.278 k D,p I8.37。推测的氨基酸序列中含有1个高度保守的NAM结构域。Gm NAC115基因组包含3个外显子和2个内含子。进化树分析发现Gm NAC115和Pv NAC为1个分支。转录活性分析结果表明,Gm NAC115转录因子具有转录激活功能。SDSPAGE电泳分析表明,p ET-28a-Gm NAC115最佳诱导表达条件为在37℃下1.0 mmol·L-1IPTG诱导2 h。组织特异性表达模式分析表明在检测的所有组织中Gm NAC115都有表达,在根中表达量最高,在种子中表达量最低。
NAC transcription factors play an important role in plant development and stress response. In this study, one NAC gene GmNAC115 was cloned from soybean. The cDNA of this gene is 1 383 bp in length and 912 bp in open reading frame, encoding 303 amino acids. The predicted molecular weight is about 34.278 kD and pI8.37. The putative amino acid sequence contains a highly conserved NAM domain. The Gm NAC115 genome contains three exons and two introns. Phylogenetic tree analysis found that Gm NAC115 and Pv NAC are one branch. The results of transcriptional activity analysis showed that Gm NAC115 transcription factor has transcriptional activation function. SDSPAGE electrophoresis analysis showed that the optimum conditions for pET-28a-Gm NAC115 induction were 1.0 mmol·L-1 IPTG induction at 37 ℃ for 2 h. Tissue-specific expression pattern analysis showed that Gm NAC115 was expressed in all the tissues tested, the highest in roots and the lowest in seeds.