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张肖冰,李慧,王凤山,等.乳腺癌细胞蛋白质O-Glc NAc糖基化水平与紫杉醇抗肿瘤作用的关系研究.中国生化药物杂志,2015,35(5):1-5.采用Weslem blot检测紫杉醇对乳腺癌细胞蛋白质0位N-酰氨基葡萄糖胺(O-GlcNAc)糖基化水平及多种相关酶蛋白表达水平的影响;RT-qPCR检测紫杉醇对N-乙酰氨基葡萄糖苷酶(NAG)和N-乙酰氨基葡萄糖转移酶(GnT)mRNA水平的影响;通过添加NAG/GnT抑制剂PUGNAc/alloxan改变蛋白质O-GlcNAc糖基化水平,测定紫杉醇对肿瘤细胞增殖的影响。结果表明紫杉
ZHANG Xiao-bing, LI Hui, WANG Feng-shan, et al. Study on the relationship between the glycosylation level of O-GlcNAc in breast cancer cells and the antitumor effect of paclitaxel. Chinese Journal of Biochemical Pharmaceutics, 2015,35 (5) The effect of paclitaxel on the level of glycosylation of O-GlcNAc at position 0 of breast cancer cells and the expression of several related enzyme proteins were detected by RT-qPCR. The effect of paclitaxel on the NAG activity of N-acetylglucosaminidase And N-acetylglucosaminyltransferase (GnT) mRNA levels. The effect of paclitaxel on the proliferation of tumor cells was assayed by changing the level of OGlcNAc glycosylation by adding NAG / GnT inhibitor PUGNAc / alloxan. The results show that yew