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以新疆乌恰县4个样地新疆沙冬青Ammopiptanthus nanus根围3个土层土壤为研究对象,利用Nested-PCR-DGGE技术,结合DGGE图谱分析、DNA克隆测序及系统发育分析,研究新疆沙冬青AM真菌菌群结构与遗传多样性特征,为新疆沙冬青根围功能菌群研究奠定基础。结果表明,不同样地和土层,AM真菌具有不同DGGE指纹图谱特征,AM真菌丰度、优势度及其多样性指数均有差异,其中上阿图什30–40cm土层AM真菌丰度和多样性指数最高,分别为20和3.48。DGGE条带测序和系统发育分析显示,全部序列归为2属14种,球囊霉属Glomus是其优势属,其中Uncultured Glomus(AB698612)和Uncultured Glomus(AB698616)普遍存在于4样地3个土层,Glomus sp.(FN429104)和Uncultured Glomus(KC797120)是上阿图什30–40cm土层特有种,Glomus sp.(EU332717),Rhizophagus intraradices(FR750206)和Uncultured Glomus(AB698616)是新疆沙冬青根围优势种。G.indicum和Rh.intraradices在形态学鉴定中未出现,说明分子生物学技术对真菌多样性研究起着补充和完善的作用。
In this study, soil samples were collected from three soils of Ammopiptanthus nanus in four sites in Wuqia County, Xinjiang Uygur Autonomous Region. Nested-PCR-DGGE, DGGE analysis, DNA sequencing and phylogenetic analysis AM fungi flora structure and genetic diversity of the characteristics of Xinjiang Ammopiptanthus mongolicum functional flora laid the foundation. The results showed that AM fungi had different DGGE fingerprinting characteristics in different plots and soil layers. The abundance, dominance and diversity index of AM fungi varied with AM fungi abundance and AM fungal abundance The highest diversity index was 20 and 3.48 respectively. DGGE sequencing and phylogenetic analysis showed that all the sequences were classified as 2 genera and 14 species. Glomus was the dominant genus, and Uncultured Glomus (AB698612) and Uncultured Glomus (AB698616) were commonly found in 4 soil samples (EU332717), Rhizophagus intraradices (FR750206) and Uncultured Glomus (AB698616) are the most common species in the region of Astragalus adsurgens Wai dominant species. G.indicum and Rh.intraradices did not appear in morphological identification, indicating that molecular biology plays a complementary and perfect role in the study of fungal diversity.