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目的 :评估临床诊断SARS患者血清特异性抗体产生规律对疾病诊断的意义。方法 :随机选取不同病程临床诊断为SARS的患者 15 6例 ,通过酶联免疫吸附的方法分别检测患者血清中特异性IgG型和IgM型抗体水平 ,分析SARS特异性抗体的产生与疾病病程的关系及对临床诊断的意义。结果 :血清IgG型抗体阳性 118例 ,阳性率为 75 .6 %。IgM型抗体阳性 6 5例 ,阳性率为 4 1.7%。IgG、IgM均为阴性 37例 ,占 2 3.7%。IgG型抗体产生阳性率随病程延长而逐渐增高 ,病程为 5 0～ 6 0d组阳性率最高达到了 94 .5 %。IgM型抗体阳性率也随病程延长而逐渐增高 ,但在病程 4 0～ 5 0d抗体阳性率即达最高 ,随后迅速下降。IgG与IgM抗体阳性率在其各自不同病程差异均十分显著 (P <0 .0 1)结论 :SARS患者血清特异性抗体检测可以作为SARS临床诊断的重要参考指标 ,但目前尚不宜作为诊断的金标准 ,SARS特异性IgG、IgM抗体同为阴性不能完全排除SARS诊断
Objective: To evaluate the significance of the law of serum-specific antibody production in the diagnosis of SARS in the diagnosis of disease. Methods: A total of 156 patients with SARS were randomly selected. The serum levels of specific IgG and IgM antibodies were detected by enzyme-linked immunosorbent assay (ELISA), and the relationship between SARS-specific antibody production and disease course was analyzed And the significance of clinical diagnosis. Results: Serum IgG antibody was positive in 118 cases, the positive rate was 75.6%. IgM antibody positive 65 cases, the positive rate of 4 1.7%. IgG, IgM were negative in 37 cases, accounting for 2 3.7%. The positive rate of IgG antibody gradually increased with the duration of the disease, the course of the disease was 50 ~ 60d group the highest positive rate of 94.5%. The positive rate of IgM antibody also increased gradually with the course of the disease, but in the course of 40 ~ 50d antibody positive rate reached the highest, then decreased rapidly. The positive rate of IgG and IgM antibody in their different durations were significantly different (P <0.01) Conclusion: SARS patients serum-specific antibodies can be used as an important reference for the clinical diagnosis of SARS, but it is not suitable for the diagnosis of gold Standard, SARS-specific IgG, IgM antibodies with the same negative can not completely rule out SARS diagnosis