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目的:研究壮药白花九里明提取物的抗菌和抗炎作用,为开发新药提供实验依据。方法:抗菌采用琼脂扩散法、肉汤稀释法测定壮药白花九里明提取物对金黄色葡萄球菌(SA)、耐甲氧西林金黄色葡萄球菌(MRSA)、铜绿假单胞菌(PA)、多重耐药铜绿假单胞菌(MDR-PA)、大肠埃希菌(E.coli)、产超广谱β-内酰胺酶大肠埃希菌(E.coli-ESBLs)、肺炎克雷伯菌(K.pne)、产超广谱β-内酰胺酶肺炎克雷伯菌(K.pne-ESBLs)的抑菌圈直径、最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。抗炎采用二甲苯致小鼠耳肿胀、醋酸致小鼠毛细血管通透性增加、角叉菜致小鼠足肿胀、琼脂致小鼠肉芽肿模型研究。结果:壮药白花九里明提取物对8种细菌具有体外抑菌活性,对SA、MRSA作用最强;水提物、醇提物对8种细菌抑菌圈直径分别是10.03~23.17mm、8.27~19.75mm;水提物对8种细菌MIC:0.020~0.691g/ml,MBC:0.025~0.758g/ml;醇提物对8种细菌MIC:0.025~1.097g/ml,MBC:0.050~1.149g/ml,水提物作用强于醇提物。水提物13.4g/kg,26.8g/kg剂量组有显著的抑制二甲苯致小鼠耳肿胀和醋酸致小鼠毛细血管通透性增高的急性炎症作用;并有明显抑制角叉菜致小鼠足肿胀、琼脂致小鼠肉芽肿的急、慢性炎症作用。结论:壮药白花九里明提取物具有广谱体外抑菌活性,对耐药菌也具有抑菌活性,水提物具有良好的抗炎作用。
OBJECTIVE: To study the antibacterial and anti-inflammatory effects of the extract of Zhuangyao Jiuliu mingli and provide experimental evidence for the development of new drugs. Methods: The antimicrobial activity of the extract of Zhujiujiujiuli on Staphylococcus aureus (SA), methicillin - resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (PA) MDR-PA, E. coli, ESBLs producing strains, Klebsiella pneumoniae (K.pne), bacteriostatic circle diameter, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of K.pne-ESBLs producing extended-spectrum β-lactamases. Anti-inflammatory Xylene-induced mouse ear swelling, acetic capillary permeability caused by mice increased, carrageenan-induced foot swelling in mice, agar-induced mouse granuloma model. Results: The extract of Zhuhuang Jiuliu Liuli had antibacterial activity in vitro against eight kinds of bacteria, and had the strongest effect on SA and MRSA. The diameters of inhibition zone of water extract and alcohol extract on the eight kinds of bacteria were 10.03 ~ 23.17mm, 8.27 ~ 19.75mm; water extract of eight kinds of bacteria MIC: 0.020 ~ 0.691g / ml, MBC: 0.025 ~ 0.758g / ml; alcohol extract against eight kinds of bacteria MIC: 0.025 ~ 1.097g / ml, MBC: g / ml, water extract stronger than alcohol extract. The aqueous extract of 13.4g / kg, 26.8g / kg dose group significantly inhibited xylene-induced mouse ear swelling and acetic acid-induced acute capillary permeability in mice increased acute inflammation; and significantly inhibited the carrageenan induced small Rodent foot swelling, agar-induced mouse granulomatous acute and chronic inflammation. Conclusion: Zhuang medicine Jiu Li Ming extract has broad-spectrum antibacterial activity in vitro, antibacterial bacteria also has antibacterial activity, water extract has a good anti-inflammatory effect.