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根据抗病基因保守序列合成7对RGAP引物扩增携带Pm 21基因的小麦-簇毛麦易位系6VS/6AL和感病品种Chancellor,结果只有R11R/R11F引物对在6VS/6AL中扩增出1 317 bp的多态性片段。在其他抗白粉病基因载体品系中检测不到该片段。回收、克隆、测序结果的Blast分析表明,该片段第1-199核苷酸、第520-792核苷酸2个区域与GenBank中的已知序列有较高的类似性,这些序列中均含有抗性蛋白的保守区域。与小麦抗叶锈病基因Lr10的序列(AY270159.1)比较结果显示,两区域的类似性达89%和86%。发现由该片段推定的氨基酸序列包含抗病基因的类似序列,有1个不完整的核苷酸结合位点(NB-ARC)。
Seven pairs of RGAP primers were synthesized based on the conserved sequence of the disease resistance genes. Wheat-cluster wheat translocation line 6VS / 6AL and susceptible variety Chancellor carrying Pm 21 gene were amplified. Only R11R / R11F primer pairs were amplified in 6VS / 6AL 1 317 bp polymorphic fragment. The fragment was undetectable in other powdery mildew resistant gene vector lines. Blast analysis of the results of recovery, cloning and sequencing showed that there were high homology between the 1-199 nucleotides and the 520-792 nucleotides of the fragment and the known sequences in GenBank, all of which contained Conserved region of the resistance protein. Compared with the sequence of wheat leaf rust resistance gene Lr10 (AY270159.1), the similarity of the two regions was 89% and 86%, respectively. The amino acid sequence deduced from this fragment was found to contain a similar sequence of disease-resistance genes with one incomplete nucleotide binding site (NB-ARC).