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目的:观察百草枯(PQ)中毒大鼠急性肺损伤模型支气管肺泡灌洗液(BALF)和血清白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平的变化及姜黄素的干预作用。方法:成年雄性SD大鼠随机分成对照组、中毒组、干预组。中毒组腹腔注射PQ 15 mg/kg,干预组腹腔注射姜黄素200 mg/kg,15 min后腹腔注射PQ 15 mg/kg,对照组腹腔注射生理盐水1 ml。各组分别按腹腔染毒或注射后3 h、6 h、24 h、3 d、7 d时间点分5个亚组。ELISA法测定各亚组大鼠BALF和血清IL-1β、TNF-α含量,光镜下观察各组大鼠肺组织病理变化。实验数据统计采用单因素方差分析,组间比较用Bonferroni法,相关性分析采用Pearson相关性检验。结果:与对照组比较,中毒组和干预组BALF和血清TNF-α、IL-1β水平在染毒后6 h、24 h、3 d、7 d均显著升高(P<0.05或P<0.01),而中毒组BALF的IL-1β水平在染毒后3 h已开始升高(P<0.01);与中毒组比较,干预组BALF和血清IL-1β和TNF-α水平在染毒后24 h、3 d、7 d均显著下降(P<0.05或P<0.01);中毒组、干预组BALF与血清IL-1β、TNF-α水平具有相关性。中毒组病理改变严重,干预组病理改变较中毒组减轻。结论:PQ中毒大鼠肺组织炎性细胞浸润,BALF和血循环中TNF-α、IL-1β等炎性因子释放增加,启动肺及全身炎症反应,可能是大鼠PQ中毒急性肺损伤的基本机制之一,姜黄素可通过此信号通路在一定程度上减轻肺损伤和全身炎性反应。
OBJECTIVE: To observe the changes of bronchoalveolar lavage fluid (BALF), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in rats with acute lung injury induced by paraquat The role of intervention. Methods: Adult male Sprague-Dawley rats were randomly divided into control group, poisoning group and intervention group. The rats in the poisoning group were injected intraperitoneally with PQ 15 mg / kg. The intervention group was given intraperitoneal injection of curcumin 200 mg / kg and the intraperitoneal injection of PQ 15 mg / kg 15 minutes later. The control group was intraperitoneally injected with 1 ml of saline. Each group was divided into 5 subgroups according to the time point of 3 h, 6 h, 24 h, 3 d and 7 d after intraperitoneal injection or injection. The contents of IL-1β and TNF-α in BALF and serum of rats in each subgroup were measured by ELISA. The pathological changes of lung tissue were observed under light microscope. Statistical analysis of the experimental data using one-way analysis of variance, comparison between groups Bonferroni method, correlation analysis using Pearson correlation test. Results: Compared with the control group, the levels of TNF-α and IL-1β in BALF and serum in the poisoning group and the intervention group were significantly increased at 6 h, 24 h, 3 d and 7 d after exposure (P <0.05 or P <0.01) ), While the levels of IL-1β in BALF of poisoning group began to increase 3 h after exposure (P <0.01). Compared with the poisoning group, the levels of IL-1β and TNF-α in BALF and serum in the intervention group were significantly increased after 24 h h, 3 d and 7 d (P <0.05 or P <0.01). The levels of IL-1β and TNF-α in BALF were significantly higher in the poisoning and intervention groups. The pathological changes in the poisoning group were serious, and the pathological changes in the intervention group were less than those in the poisoning group. CONCLUSION: Inflammatory cytokines infiltration in the lungs of PQ-poisoning rats and the release of inflammatory cytokines such as TNF-α and IL-1β in the BALF and blood circulation are increased, and lung and systemic inflammatory reactions are initiated, which may be the basic mechanism of PQ intoxication in acute lung injury One, curcumin can reduce lung injury and systemic inflammatory response through this signaling pathway to a certain extent.