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目的:分析染色体6q2 1的D6S4 4 9 D6S30 2区域等位基因杂合性缺失的情况,确定其在中国儿童急性淋巴细胞性白血病中发生的频率和最小缺失区域,为定位克隆ALL相关抑癌基因提供线索。方法:采用比以往报道更高密度的11个微卫星多态性标记位点,利用聚合酶链反应 变性凝胶电泳银染技术分析93例儿童急性淋巴细胞性白血病例等位基因LOH的情况。结果:93例患者中共有32例(34.4 % )存在至少一个位点的LOH。位点D6S170 9 D6S30 1以及位点D6S30 2是高频率缺失集中的区域,位于其中的D6S2 83位点附近是缺失率最高的地方(2 5 % )。D6S170 9 D6S30 1之间距离在GenethonMap上显示有2cM。结论:6S170 9 D6S30 1为本研究所找到的6q2 1上的最小缺失区域,当中可能存在与中国儿童急性淋巴细胞性白血病发病相关的一个或一个以上的候选抑癌基因
OBJECTIVE: To analyze the loss of allelic heterozygosity in the D6S4 4 9 D6S30 2 region of chromosome 6q2 1, and to determine the frequency and the region of minimal deletions in Chinese children with acute lymphoblastic leukemia. In order to locate the associated tumor suppressor gene ALL Provide clues. METHODS: Eleven microsatellite loci with higher density than those previously reported were used to analyze the allele LOH in 93 children with acute lymphoblastic leukemia using polymerase chain reaction-denaturing gel electrophoresis with silver staining. RESULTS: A total of 32 of 93 patients (34.4%) had LOH at at least one locus. Loci D6S170 9 D6S30 1 and D6S30 2 are the regions with high frequency loss concentration, and the highest deletion rate (25%) is located in the vicinity of the D6S2 83 locus. The distance between D6S170 9 D6S30 1 shows 2cM on GenethonMap. Conclusion: 6S170 9 D6S30 1 is the smallest deletion region in 6q2 1 found in this study, in which there may be one or more candidate tumor suppressor genes associated with the onset of childhood acute lymphoblastic leukemia in children